| HPLC法测定母姜与子姜中的6-姜酚,8-姜酚和10-姜酚 |
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| 引用本文： | 王丽,徐美霞,王梅,王晓,刘建华.HPLC法测定母姜与子姜中的6-姜酚,8-姜酚和10-姜酚[J].山东科学,2013,26(1):12-15. |
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| 作者姓名： | 王丽 徐美霞 王梅 王晓 刘建华 |
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| 作者单位： | 1.山东省分析测试中心，山东 济南 250014；
2.山东农业大学食品科学与工程学院，山东 泰安 271018；
3.山东师范大学生命科学学院，山东 济南 250014 |
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| 基金项目： | 山东省科技攻关项目（2010GSF10287） |
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| 摘 要： | 利用高效液相色谱（HPLC）法测定母姜与子姜中6 姜酚、8 姜酚和10 姜酚的含量。采用Inertsil ODS-SP色谱柱（250 mm×4.6 mm, 5 μm）分析，二元线性梯度洗脱，紫外检测器在280 nm波长下检测。结果表明本实验样品母姜中6 姜酚、8 姜酚和10 姜酚含量分别是子姜的2倍、1.5倍和2倍。
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| 关 键 词： | 高效液相色谱 母姜 子姜 6-姜酚 8-姜酚 10-姜酚 |
| 收稿时间： | 2012-04-11 |
High performance liquid chromatography based determination of6-gingerol,8-gingerol and 10-gingerol in seed and tender ginger |
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| WANG Li
,
XU Mei-xia
,
WANG Mei
,
WANG Xiao
,
LIU Jian-hua.High performance liquid chromatography based determination of6-gingerol,8-gingerol and 10-gingerol in seed and tender ginger[J].Shandong Science,2013,26(1):12-15. |
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| Authors: | WANG Li XU Mei-xia WANG Mei WANG Xiao LIU Jian-hua |
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| Institution: | 1. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China;
2. School of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China;
3. School of life Sciences, Shandong Normal University, Jinan 250014, China |
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| Abstract: | We employed high performance liquid chromatography (HPLC) to determine the contents of 6-, 8-, and 10-gingerol in seed and tender ginger. The determination conditions were an Inertsil ODS-SP column (250 mm×4.6 mm, 5 μm), a binary eluent under gradient condition and ultraviolet detection at 280 nm wavelength. Experimental results show that the contents of 6-, 8- and 10-gingerol in seed ginger are separatively two times, one and a half times and two times of those in tender ginger. |
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| Keywords: | HPLC seed ginger tender ginger 6-gingerol 8-gingerol 10-gingerol |
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