山梗菜嫩茎无性系的建立

以山梗菜的嫩茎为试验材料，先后对其愈伤组织培养、愈伤组织分化、分化芽生根和试管苗的栽培进行了研究．结果证明：MS＋ KT0．2mg·L -1＋2，4-D1．4mg·L -1是愈伤组织诱导培养的理想培养基；1/2MS＋ NAA0．1 mg · L -1＋ZT0．1 mg · L -1＋AgNO30．3 mg · L -1是愈伤组织分化培养的理想培养基；1/2MS＋NAA0．1 mg · L -1＋IAA0．2 mg · L -1＋ABT2号1．0 mg · L -1是试管苗生根继代增殖培养和不定芽生根培养的适宜培养基．试管苗移栽成活率为94．2％；定植成活率达到了99％以上．定植的试管苗不仅具有野生山梗菜所有植物学性状，而且还能在翌年正常开花结果．

Study on clone establishment of Lobelia sessili f olia

With the method of tissue culture ,tender stems of Lobelia sessili f olia were used as material to do the research on callus inducement and differentiation ,rooting of adventitious buds ,ransplanta-tion of tube seedlings and stable planting .MS＋KT0 .2 mg · L -1 ＋2 ,4-D1 .4 mg · L -1 was the ideal medium for callus inducing ;1/2MS＋NAA0 .1 mg · L -1 ＋ZT0 .1 mg · L -1 ＋ AgNO30 .3 mg · L -1 was suitable for callus differentiation ;the best medium for rooting of adventitious buds and rooting multiplication was 1/2MS＋No .ABT2 1 .0 mg · L -1 ＋ NAA0 .1 mg · L -1 ＋ IAA0 .2 mg · L -1 .The transplanting survival rate of tube seedlings was 94 .2% and stable planting survival rate was 99% . The tube seedlings stable planted maintained all botany characteristics of the wild Lobelia sessili f olia and beared following year .