| 用壳聚糖修饰的MCM-48固定化脂肪酶拆分（R，S）-1-苯乙醇 |
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| 引用本文： | 严祥辉,薛屏.用壳聚糖修饰的MCM-48固定化脂肪酶拆分（R，S）-1-苯乙醇[J].宁夏大学学报(自然科学版),2009,30(1):50-54. |
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| 作者姓名： | 严祥辉 薛屏 |
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| 作者单位： | 宁夏大学,能源化工重点实验室,宁夏,银川,750021 |
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| 摘 要： | 研究了涂覆壳聚糖（CS）的介孔分子筛MCM-48固定化假单胞菌脂肪酶（PSL），及其在（R，S）-1-苯乙醇转酯化拆分反应中的催化性能．结果表明，壳聚糖与MCM-48的质量比为1：10时，用有机相固定化法制备的固定化酶PsL／CS-MCM-48显示了较高的催化活性及对映选择性，且高于游离酶和纯壳聚糖固定化酶PSL/CS．在PSL／CS-MCM-48的催化作用下，1-苯乙醇的转化率（C）达到46．9％，（S）-1-苯乙醇的对映体过量值（ees）达到87．5％，产物（R）-1-乙酸苯乙酯的对映体过量值（eep）大于99％，对映选择性参数（E）为576．同时，PSL／CS-MCM-48对（R，S）-1-苯乙醇催化转酯化拆分具有良好的重复使用性．
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| 关 键 词： | 固定化脂肪酶 壳聚糖修饰 MCM-48 手性拆分 (R S)-1-苯乙醇 |
Chiral Resolution of(R,S)-1-phenylethanol Catalyzed by Lipase Immobilized on MCM-48 Coated Chitosan |
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| Yan Xianghui,Xue Ping.Chiral Resolution of(R,S)-1-phenylethanol Catalyzed by Lipase Immobilized on MCM-48 Coated Chitosan[J].Journal of Ningxia University(Natural Science Edition),2009,30(1):50-54. |
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| Authors: | Yan Xianghui Xue Ping |
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| Institution: | Key Laboratory of Energy Resources and Chemical Engineering;Ningxia University;Yinchuan 750021;China |
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| Abstract: | The catalytic performance of Pseudomonas cepacia lipase （PSL） immobilized on the mesoporous molecular sieve MCM-48 coated chitosan （CS） in the transesterification resolution of （R, S）-l-phenylethanol was studied. The results showed that adjusting the mass ratio of CS and MCM-48 to 1 - 10, the immobilized lipase PSL/CS-MCM-48 prepared in organic solvent exhibited high catalytic activity and enantioselectivity, which outclassed that of free lipase and PSL/CS. 87. 5 % enantiomeric excess （ees） of substrate （S）-l-phenylethanol, 99% enantiomeric excess（eep） of （R）-1-phenylethyl acetate with 46.9% conversion and enantioselectivity E value of 576 were obtained by PSL/CS-MCM-48. The PSL/CS-MCM-48 also displayed the excellent reusability which was obviously superior to that of the free lipase and PSL/CS. |
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| Keywords: | MCM-48 |
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