老年急性冠脉综合征患者的细胞免疫功能变化

目的:通过检测老年急性冠脉综合征(ACS)病人血清中的细胞因子γ-干扰素(IFN-γ),免疫细胞表面抗原分子CD3~+、CD4~+、CD8~+的浓度水平,探讨老年ACS病人的细胞免疫功能改变.方法:36例ACS患者为实验组,40例正常对照组老年人的外周静脉血,酶联免疫吸附法(ELISA)测定IFN-γ含量,采用碱性磷酸酶-抗碱性磷酸酶桥联酶染色法(APAAP)测定T淋巴细胞亚群细胞数.结果:实验组IFN-γ含量和CD3~+、CD4~+、CD4~+/CD8~+比值低于对照组(P=0.001),而CD8~+则高于对照组(P=O.001),差异均有显著性意义.结论:老年ACS患者体内细胞免疫功能的改变可能对ACS的形成和发展产生了一定的影响,T淋巴细胞亚群比值和IFN-γ含量变化可作为老年ACS严重程度的监测指标.

Study on variation of cellular immunity in senior patients of acute coronary syndromes

Aim:To investigate change of cellular immunity in senior patients of ante coronary syn-dromes via evaluating the serum level of IFN-γ and CD3~+,CD4~+ ,CD8~+. Methods: The level of IFN-γ was measured by ELISA, and the level of T-lymphocyte subpopulations in blood was assayed by APAAP method from 36 senior patients with ACS, forty healthy old persons as controls (NC). Results: The level of CD3~+,CD4~+,IFN-γ, and the ratio of CD4~+/CD8~+in group ACS were less than those in group NC(P=0.001 ). But the level of CD8~+was more than that in group NC(P=0.001 ). Conclusion: The im-mune change in ACS has an effect on forming and progress of ACS. The ratio of T-lymphocyte subpopula-tions and the level of IFN-γ in blood can be the measure to detect the serious degree of ACS.