Analysis of IGF2 mRNA expression and its methylation status between cattle yaks and their parents |
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| Authors: | Zhenshan Liu Qifa Li Qingbo Zhang Xuguang Qu Liyan Dong Chamba Yang-zom Zhuang Xie Honglin Liu |
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| Institution: | aLaboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;bInstitute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China;cCollege of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000, China |
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| Abstract: | Cattle yaks are an F1 hybrid between cattle and yaks and exhibit significant hybrid vigor. However, cattle yaks are sterile males. To study the epigenetic aspects of this reproductive isolation, IGF2 mRNA expression in cattle yaks and their parents and the patterns of the IGF2 differentially methylated regions (DMR) were examined in blood and testes. The results showed that IGF2 expression in cattle yaks’ testes was lower than in their parents (P < 0.01). The IGF2 DMR was highly methylated (above 90%) in blood and testes of cattle yaks, yaks and cattle, with the highest level in cattle yaks (P > 0.05). Our study showed that IGF2 plays an important role in bovine spermatogenesis and might be involved in cattle-yak male sterility. The methylation level of the IGF2 DMR was irrelevant to the lower expression of IGF2 in cattle yaks; other factors perhaps play roles in its expression. |
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| Keywords: | Cattle yak Male sterility IGF2 gene mRNA expression DNA methylation |
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