| 两种缓冲系统对鱼类乳酸脱氢酶(LDH)同工酶分离效果的比较研究 |
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| 作者单位： | 福建师范大学生物工程学院 福州350007 |
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| 摘 要： | 采用pH8.3 Tris—Gly缓冲系统和pH8.4 EBT缓冲系统的聚丙烯酰胺凝胶电泳对大鳞副泥鳅和草鱼各组织的乳酸脱氢酶同工酶进行了分离比较,发现采用EBT缓冲系统分离效果较好,大鳞副泥鳅各组织可分离到10条酶带,草鱼各组织可分离到20多条酶带.
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| 关 键 词： | 大鳞副泥鳅 草鱼 LDH同工酶 Tris—Gly缓冲系统 EBT缓冲系统 |
COMPARISON OF THE EFFECT ON APPLICATION WITH TWO KINDS OF BUFFER SYSTEM TO RESOLVE FISH LDH ISOZYMES |
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| Authors: | Xiao Yijun Zhang Qujin |
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| Abstract: | The LDH isozymes in Paramisgurnus dabryanus and Grass Carp are resolved by Poeyacrylamide gel electrophoresis with pH8. 3 Tris-Gly buffer and pH8. 4 EBT buffer. The result shows that EBT buffer is more effective. There are ten LDH isozymes bands resolved from the tissues of Paramis gurnus dabryanus and more than twenty LDH isozymes bands resolved from the tissues of Grass Carp. |
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| Keywords: | Paramis gurnus dabryanus Grass Carp LDH isozymes Tris-Gly buffer EBT buffer |
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