| 高粱色素含量测定方法的研究 |
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| 引用本文： | 毛绍春,李竹英.高粱色素含量测定方法的研究[J].云南大学学报(自然科学版),2011(Z1). |
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| 作者姓名： | 毛绍春 李竹英 |
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| 作者单位： | 玉溪农业职业技术学院; |
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| 基金项目： | 云南省教育厅科学研究基金资助项目(08Y0387); 玉溪农业职业技术学院科学研究基金资助 |
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| 摘 要： | 用光度法研究了2种测定高粱色素含量的方法.一种是利用高粱色素溶液在494 nm处有最大吸收峰进行含量测定(直接测定法);另一种是在高粱色素溶液中加入显色剂后,在510 nm处有最大吸收峰而进行含量测定(间接测定法).结果表明,对于理论含量为20.0 g/L高粱色素溶液,用直接法和间接法测定的质量浓度分别为19.44 g/L和18.40 g/L,相对标准差分别为0.46%和3.37%,加标回收率分别为98.83%和88.58%.直接测定法具有快速、准确的特点.
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| 关 键 词： | 高粱色素 测定 光度法 |
Study on determination method of content of sorghum pigment |
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| MAO Shao-chun,LI Zhu-ying.Study on determination method of content of sorghum pigment[J].Journal of Yunnan University(Natural Sciences),2011(Z1). |
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| Authors: | MAO Shao-chun LI Zhu-ying |
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| Institution: | MAO Shao-chun,LI Zhu-ying(Yuxi Agricultural Vocational Institute,Yuxi 653100,China) |
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| Abstract: | Two kinds of determination methods of content of sorghum pigment were studied by spectrophotometry.The first method: sorghum pigment solution has maximum absorption peak at 494 nm(direct method).The second,the mixed solition,which chromogenic agent was added to sorghum pigment solution,has maximum absorption peak at 510 nm(indirect method).The theoretical content of 20.0 g/L sorghum pigment solution was determined by direct method and indirect method.The determination content of sorghum pigment are 19.44 g/... |
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| Keywords: | sorghum content determination spectrophotometry |
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