| 高效液相色谱法测定五香血藤中五内酯E |
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| 引用本文： | 干正洋,杨庆雄.高效液相色谱法测定五香血藤中五内酯E[J].云南大学学报(自然科学版),2011,0(5):590-593. |
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| 作者姓名： | 干正洋 杨庆雄 |
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| 作者单位： | 贵州师范大学化学与材料科学学院;贵州师范学院化学与生命科学学院; |
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| 基金项目： | 贵州省教育厅自然科学研究项目(黔教科20090127)资助 |
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| 摘 要： | 采用HPLC法测定五香血藤中五内酯E的含量.色谱柱:sphenrisorb C18(4.0 mm×250 mm,5μm),流动相:A水,B乙腈,按体积分数梯度洗脱70%～90%,0～25 min,波长210 nm.线性范围为1.00～5.00μg·mL-1,相关系数R2=0.999 9,平均回收率为97.73%,相对标准偏(RSD)为2.33%.方法具有简単、灵敏、准确等特点.使用该方法测定了贵州8个不同产地的五香血藤的含量,结果满意.
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| 关 键 词： | 五香血藤 五内酯E 高效液相色谱 含量测定 |
Determination of schisanlactone E in Kadsara longipedunculata by HPLC |
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| GAN Zheng-yang,YANG Qing-xiong.Determination of schisanlactone E in Kadsara longipedunculata by HPLC[J].Journal of Yunnan University(Natural Sciences),2011,0(5):590-593. |
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| Authors: | GAN Zheng-yang YANG Qing-xiong |
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| Institution: | GAN Zheng-yang1,2,YANG Qing-xiong1(1.School of Chemistry & Materials Sciences,Guizhou Normal University,Guiyang 550001,China,2.School of Chemistry Life Sciences,Guizhou Normal College,Guiyang 550018,China) |
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| Abstract: | To establish a HPLC method for determination of schisanlactoneE in Kadsara longipedunculata.Sphenrisorb-C18 column was used.The mobile phase consisted of 70%—90% CH3CN-H2O.Detection wavelength was set at 210 nm.The linear range was 1.00—5.00 μg and the correlation coefficient(R2) was 0.999 9.The average recovery was 97.73%.The relative standard deviation(RSD) was 2.33%.The method is simple,sensitive and accurate.Eight kinds of Kadsura longipedunculata were determined and the results were satisfactory. |
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| Keywords: | Kadsura longipedunculata schisanlactone E HPLC determination |
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