| 不同培养基和生长调节剂对细叶小羽藓生长发育及愈伤组织诱导的影响 |
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| 引用本文： | 饶本强,朱亚利,杨淑岚,张晨迎,余亚军,马尹尹.不同培养基和生长调节剂对细叶小羽藓生长发育及愈伤组织诱导的影响[J].信阳师范学院学报(自然科学版),2019(1):47-52. |
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| 作者姓名： | 饶本强 朱亚利 杨淑岚 张晨迎 余亚军 马尹尹 |
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| 作者单位： | 信阳师范学院生命科学学院/河南省茶树生物学重点实验室/大别山农业生物资源保护与利用研究院;信阳市林业工作站 |
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| 摘 要： | 对淮河上游南湾湖周边的细叶小羽藓(Haplocladium microphyllum)的孢子生长发育进行观察,发现细叶小羽藓孢子无休眠现象,孢子接种2天左右萌发;扩大培养实验结果表明,细叶小羽藓无菌原丝体在1/2 MS培养基上置于20℃±2℃、12 h光照/12 h黑暗条件培养,产生配子体最多,且配子体长势最好,可以获得大量无菌材料;细叶小羽藓愈伤组织诱导实验显示,形成愈伤组织的最佳培养基为添加1%葡萄糖+1.5 mg/L6-BA+0.25 mg/L 2,4-D的MS培养基.
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| 关 键 词： | 细叶小羽藓 生长发育 愈伤组织诱导 培养基 生长调节剂 |
Effects of Different Culture Medium and Growth Moderating Substances on Growth and Callus Induction of Haplocladium Microphyllum |
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| Institution: | ,College of Life Sciences/Key Laboratory of Tea Plant Biology of Henan Province/Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains,Xinyang Normal University,Forestry Workstation in Xinyang |
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| Abstract: | Based on observation of the growth and development of spores of Haplocladium microphyllum in the south of Nanwan lake of Xinyang,it was found that there was no dormancy in the sphagnum spore,and the spore inoculation was germinated about 2 days. Expand training experimental results showed that the fine Haplocladium microphyllum sterile protonema moss on the 1/2 of the MS culture medium at 20 ℃ ±2 ℃,12 h of light/dark conditions to cultivate,produce new stem leaf body up,and the stem leaf do best,get a lot of aseptic materials; The experimental results showed that the optimal medium for the formation of callus was 1% glucose + 1.5 mg/L 6-BA+0.25 mg/L 2,4-D MS medium. |
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| Keywords: | Haplocladium microphyllum growth development callus induction culture medium growth moderating substances |
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