| 秀丽线虫对根际益生菌P13和S3-1传播作用及对植物生长的影响 |
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| 引用本文： | 刘星星,杨文武,陈佳佳,肖明.秀丽线虫对根际益生菌P13和S3-1传播作用及对植物生长的影响[J].上海师范大学学报(自然科学版),2017,46(4):460-468. |
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| 作者姓名： | 刘星星 杨文武 陈佳佳 肖明 |
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| 作者单位： | 上海师范大学 生命与环境科学学院, 上海 200234,上海师范大学 生命与环境科学学院, 上海 200234,上海师范大学 生命与环境科学学院, 上海 200234,上海师范大学 生命与环境科学学院, 上海 200234 |
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| 基金项目： | 上海市2016年度"科技创新行动计划"项目（16391902100） |
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| 摘 要： | 通过缓慢杀线实验、偏好性实验和共培养毒性实验分析了秀丽线虫(Caenorhabditis elegans)对荧光假单胞菌P13(Pseudomonas fluorescens P13)和解淀粉芽孢杆菌S3-1(Bacillus amyloliquefaciens S3-1)传播的可能性.通过显微观察与平板稀释涂布对秀丽线虫细菌携带作用进行了定性、定量分析,并对细菌—线虫—植物三者交互作用进行初步探究.结果发现,P13和S3-1对秀丽线虫的慢性致死率分别为12.12%和3.00%,每10 s身体弯曲次数分别为4.68和4.33.相对于尿嘧啶缺陷型大肠杆菌(OP50),线虫对P13和S3-1选择系数分别为0.13和0.52,P13、S3-1携带菌量分别为(4.02×103±47)和(9.67×102±22)CFU/条.携细菌线虫将细菌定向传播至植物根际,细菌定殖菌量为105CFU,有效促进植物生长发育.
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| 关 键 词： | 秀丽线虫 根际益生菌 荧光假单胞菌P13 解淀粉芽孢杆菌S3-1 |
| 收稿时间： | 2016/7/3 0:00:00 |
The effects of Caenorhabditis elegans on plant growth-promoting rhizobacteria and plant growth |
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| Liu Xingxing,Yang Wenwu,Chen Jiajia and Xiao Ming.The effects of Caenorhabditis elegans on plant growth-promoting rhizobacteria and plant growth[J].Journal of Shanghai Normal University(Natural Sciences),2017,46(4):460-468. |
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| Authors: | Liu Xingxing Yang Wenwu Chen Jiajia and Xiao Ming |
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| Institution: | College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China and College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China |
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| Abstract: | The diversity and ecology functions of soil nematodes have gained comprehensive attentions.In this study,we conducted a series of experiments (the slow killing assay,long time toxicity experiment and preference experiment)to test whether nematodes Caenorhabditis elegans(C.elegans) may contribute to the spread of the P13 and S3-1.Combining microscopic observation with dilution-plate method,we analyzed the bacteria carried by C.elegans.Furthermore,we preliminarily explored the interactions of bacteria,nematodes and plants.Death rates of C.elegans in slow killing assay driven by two strains were 12.12% (P13) and 3.0% (S3-1),separately.The body bending frequencies of C.elegans were 4.68/10s (P13) and 4.33/10s (S3-1),respectively.Choice indexes of P13 and S3-1 were 0.13 and 0.52,separately.The total numbers of bacteria carried by C.elegans were 4.02×103±47 (P13) and 9.67×102±22 (S3-1).C.elegans carried bacteria to the rhizosphere where the bacteria content was as high as 105 CFU per plant root.As a vector of rhizosphere bacteria,C.elegans can carry P13 and S3-1 to plant rhizosphere and effectively promote plant growth and development. |
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| Keywords: | Caenorhabditis elegans plant growth-promoting rhizobacteria Pseudomonas fluorescens P13 Bacillus amyloliquefaciens S3-1 |
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