Isolation and identification of the three rice monotelosomics

In order to obtain rice monotelosomic, the progeny of 24 telotrisomics, derived from an indica rice variety, Zhongxian 3037, were screened. The variants that differed morphologically from the diploids and the original primary trisomics as well as the telotrisomics were collected for cytological identification. The variants with 24 chromosomes were selected according to the prometaphase chromosomes. From these variants, three monotelosomies with one chromosome arm deletion in each were verified by fluorescence in situ hybridization （FISH） using a rice centromeric BAC clone of 17p22 as a marker probe. The three monotelosomics were derived from telotrisomic 1S, 4L and 11L, respectively. Further identification was conducted on the prometaphase or pachytene chromosomes of the three variants, which were probed with the same centromeric BAC clone together with the corresponding chromosome arm specific makers, a0059H02 （on the short arm of chromosome 1）, a0034E24 （on the long arm of chromosome 4）, and a0071H11 （on the long arm of chromosome 11）. The results indicated that the telocentric chromosomes in the three monotelosom. ics were derived from their respective corresponding telotrisomics. According to the telocentric chromosomes of the variants, they were monotelosomic 1S （one long arm of chromosome 1 was lost）, monotelosomic 4L （one short arm of chromosome 4 was lost） and monotelosomic 11L （one short arm of chromosome 11 was lost）, respectively.

Isolation and identification of the three rice monotelosomics

In order to obtain rice monotelosomic, the progeny of 24 telotrisomics, derived from an indica rice vari-ety, Zhongxian 3037, were screened. The variants that dif-fered morphologically from the diploids and the original primary trisomics as well as the telotrisomics were collected for cytological identification. The variants with 24 chromo-somes were selected according to the prometaphase chromo-somes. From these variants, three monotelosomics with one chromosome arm deletion in each were verified by fluores-cence in situ hybridization (FISH) using a rice centromeric BAC clone of 17p22 as a marker probe. The three monote-losomics were derived from telotrisomic 1S, 4L and 11L, re-spectively. Further identification was conducted on the pro-metaphase or pachytene chromosomes of the three variants, which were probed with the same centromeric BAC clone together with the corresponding chromosome arm specific makers, a0059H02 (on the short arm of chromosome 1), a0034E24 (on the long arm of chromosome 4), and a0071H11 (on the long arm of chromosome 11). The results indicated that the telocentric chromosomes in the three monotelosom-ics were derived from their respective corresponding telotrisomics. According to the telocentric chromosomes of the variants, they were monotelosomic 1S (one long arm of chromosome 1 was lost), monotelosomic 4L (one short arm of chromosome 4 was lost) and monotelosomic 11L (one short arm of chromosome 11 was lost), respectively.