1株碱杆菌属菌株的分离和鉴定

 从新疆采集的盐土样品中分离到1株革兰氏阳性、严格好氧、产孢、嗜盐细菌菌株YIM 012,对其进行了系统分类学研究.菌株YIM 012为杆菌,生长最适温度为28~45℃,最适pH 7.0~8.0,并能在70~250g/L的盐质量浓度中生长良好.其DNA的G+C的摩尔分数为39%,16S rRNA基因序列分析结果显示,该菌株与碱杆菌属Alkalibacillus salilacus(DSM 16460^T)亲缘关系最近,相似性达到99.6%,但与同属其它2个有效种间相似性低于96%.杂交结果显示其与A. salilacus亲缘关系低于20%,并且在生理生化方面也有差异.综合分析,确定该菌株为碱杆菌属的1个新种,将其命名为嗜盐碱杆菌(Alkalibacillus halophilus).

Isolation and identification one Alkalibacillus strain

A spore-forming,gram positive Bacillus-like,strain YIM 012,was isolated from one hypersaline soil sample collected from Xinjiang Province,China and was subjected to a polyphasic taxonomy study.The isolated strain was motile,aerobic and appearing as halophilic in nature,based on its optimum growth condition between 70—250 g/L of NaCl concentration.The genomic DNA G+C content of the strain was 39 mol%.Phylogenetic analysis based on 16S rRNA gene sequences showed that the closest relative of the strain was(Alkalibacillus salilacus)(99.6%)and the relatedness value of DNA-DNA hybridization was of 20%.Their physiological and biochemical characterizations were different in many aspects.Therefore,on the basis of polyphasic taxonomic data presented,a novel species of the genus Alkalibacillus,Alkalibacillus halophilus sp.nov,was proposed.The type strain was YIM 012.