| 不动杆菌D10对土壤中对硫磷的降解 |
| |
| 引用本文： | 姜莉,史艳芳,刘鑫,李志明,张雪雨,姜彬慧.不动杆菌D10对土壤中对硫磷的降解[J].科技导报(北京),2011,29(13):33-39. |
| |
| 作者姓名： | 姜莉 史艳芳 刘鑫 李志明 张雪雨 姜彬慧 |
| |
| 作者单位： | 1. 沈阳出入境检验检疫局，沈阳 110016;2. 东北大学资源与土木工程学院，沈阳 110004 |
| |
| 基金项目： | 2008年辽宁省科技计划重大、重点项目(2008215002); 2008沈阳市大型科学仪器设备共享服务专项(2008gx-019) |
| |
| 摘 要： | 为了考查微生物对农药污染土壤的修复作用，本文从受农药污染的土壤中筛选出一株对对硫磷有降解作用的菌株D10。采用室内培养方法，根据其形态特征、生理生化特性和16S rDNA序列分析，鉴定D10为不动杆菌属（Acinetobacter sp.）的微生物。通过分光光度比浊法研究其最佳生长条件，结果表明，D10的最佳生长温度为28℃，最佳培养初始pH值为6.0—7.0。D10生长周期较短，在营养培养基中培养24h就可达到最大生物量，其培养的最佳C、N源，分别为柠檬酸钠和酵母粉。利用气相色谱检测菌株D10对对硫磷的降解作用，其24h的对硫磷降解率达到83.1%。通过对模拟污染土壤中对硫磷的降解效果的研究，结果表明，在农药污染的土壤中对硫磷浓度为100mg/kg时，D10对对硫磷农药7d的降解率达到66.7%，显示D10对对硫磷污染土壤具有较强的修复作用。
|
| 关 键 词： | 环境生物学 对硫磷 不动杆菌属 生物降解 |
Biodegradation of Parathion in Soil by Acinetobacter sp. D10 |
| |
| JIANG Li,SHI Yanfang,LIU Xin,LI Zhiming,ZHANG Xueyu,JIANG Binhui .Shenyang Entry-Exit Inspection , Quarantine Bureau,Shenyang ,China .College of Resources , Civil Engineering,Northeastern University,Shenyang ,China.Biodegradation of Parathion in Soil by Acinetobacter sp. D10[J].Science & Technology Review,2011,29(13):33-39. |
| |
| Authors: | JIANG Li SHI Yanfang LIU Xin LI Zhiming ZHANG Xueyu JIANG Binhui Shenyang Entry-Exit Inspection Quarantine Bureau Shenyang China College of Resources Civil Engineering Northeastern University Shenyang China |
| |
| Institution: | 1. Shenyang Entry-Exit Inspection and Quarantine Bureau, Shenyang 110016, China;2. College of Resources and Civil Engineering, Northeastern University, Shenyang 110004, China |
| |
| Abstract: | Organophosphorus pesticides are worldwide widely used to control agricultural and household pests.Overall,organophosphorus compounds account for the 38% of total pesticides used globally.Parathion is a virulent organophosphate insecticide with perfect insecticidal efficiency.However,it is also highly toxic and harmful to human and other creatures.In order to investigate microbial remediation of soil polluted by pesticides,a strain D10 of parathion-degrading is obtained by selective enrichment culture from t... |
| |
| Keywords: | environmental biology parathion Acinetobacter sp biodegradation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《科技导报(北京)》浏览原始摘要信息 |
| 点击此处可从《科技导报(北京)》下载免费的PDF全文 |
|
