| 酿酒酵母的双亲株原生质体灭活融合研究 |
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| 引用本文： | 李潞滨,朱宝成,王俊刚,李庆余.酿酒酵母的双亲株原生质体灭活融合研究[J].河北大学学报(自然科学版),1991(1). |
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| 作者姓名： | 李潞滨 朱宝成 王俊刚 李庆余 |
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| 作者单位： | 河北大学生物系
(李潞滨，朱宝成，王俊刚)，河北大学生物系(李庆余) |
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| 摘 要： | 把两株酿酒酵母(saccharomyces cerevisiae)S_1、S_2的原生质体分别用紫外线(U.V)和碘乙酰胺灭活或只用紫外线灭活,经PEG诱导融合后,在完全培养基上得到了融合子。融合频率为(0.98-2.13)×10~(-7)。融合子的细胞形态、DNA含量等都和亲株有明显差别,并在生产特性上有某些改进。
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| 关 键 词： | 酿酒酵母 原生质体灭活 融合 |
Fusion of Inactivated Protoplasts of Saccharomyces Ccrevisiae |
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| Li Lubin Zhu Baotheng Wang Zungang Li Qingyu.Fusion of Inactivated Protoplasts of Saccharomyces Ccrevisiae[J].Journal of Hebei University (Natural Science Edition),1991(1). |
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| Authors: | Li Lubin Zhu Baotheng Wang Zungang Li Qingyu |
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| Institution: | Department of Biology |
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| Abstract: | Protoplasts of S. ccrcvisiac were treated with 0.85% Todoacctamidc for 10 minutes lost the ability to form colonies, whereas ultraviolet irradiation of the protoplasts suspension for 6-9 minutes gave a survival, which ranged from 0 to 10-7When the U.V-inactivated protoplasts of one parent strain Fused with another U.V-or lodoacctamidc treated strain, the frequencies ariscd in the fusants were (0.98-2.31) x10-7. The results showed the possibility that in strain improvment of industrial microorganisms hybrid strains might be isolated without introducing genetics markers into any parent strain. |
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| Keywords: | S ccrcvisiac protoplasls inaclivalion fusion |
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