| 中国人磷酸核糖焦磷酸合成酶-1假基因的克隆与测序 |
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| 引用本文： | 薛爱群,张宏,王晓卿.中国人磷酸核糖焦磷酸合成酶-1假基因的克隆与测序[J].中山大学学报(自然科学版),2000,39(3):135-136. |
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| 作者姓名： | 薛爱群 张宏 王晓卿 |
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| 作者单位： | 中山大学生物工程研究中心!广东广州510275 |
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| 基金项目： | 国家自然科学基金资助项目!(39770 354) |
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| 摘 要： | 自从第一个假基因被鉴定以后1] ,有关假基因的性质与发生正在被阐明 .假基因基本上分为两类 :非加工的假基因和被加工的假基因 .被加工的假基因通常与活跃基因序列比较同源性高达 90 %～ 99% ,不含内含子 ,具有ｐｏｌｙ (Ａ)尾巴 ,可以转录 ,但由于在它们内部有许多终止子、插
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| 关 键 词： | 假基因 DNA测序 磷酸核糖焦磷酸合成酶 基因克隆 |
Cloning and Sequencing of Phosphoribosylpyrophosphate Synthetase-1 Processed Peseudogene in Chinese |
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| XUE Ai qun,ZHANG Hong,WANG Xiao qing,FANG Xiang ping,ZHOU QinBiotechnologyResearchCenter,ZhongshanUniversity,Guangzhou ,China.Cloning and Sequencing of Phosphoribosylpyrophosphate Synthetase-1 Processed Peseudogene in Chinese[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2000,39(3):135-136. |
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| Authors: | XUE Ai qun ZHANG Hong WANG Xiao qing FANG Xiang ping ZHOU QinBiotechnologyResearchCenter ZhongshanUniversity Guangzhou China |
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| Institution: | XUE Ai qun,ZHANG Hong,WANG Xiao qing,FANG Xiang ping,ZHOU QinBiotechnologyResearchCenter,ZhongshanUniversity,Guangzhou 510 2 75,China |
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| Abstract: | A processed pseudogene cDNA of PRS1 was first cloned from lymphoblast cells of normal persons and primary gout patients among Chinese. The full length of the pseudogene cDNA was 985 bp, which possessed approx. 89 7% homology with the published sequence. Except many point mutations, two frame shift deletion of 5 bp and 8 bp were also found at the location 366 bp and 648 bp of the pseudogene cDNA, respectively. All led to 13 stop codons, so that fragment can't translate. According to these characteristics, the fragment was thought as a processed pseudogene of PRS1. |
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| Keywords: | PRS1 pseudogene DNA sequence and analysis |
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