熏烟加寒燥环境对大鼠基质金属蛋白酶及气道细胞外基质成分的影响

 为了探讨熏烟加新疆寒燥环境对大鼠血清基质金属蛋白酶-9(MMP-9)和肿瘤坏死因子-α(TNF-α)、肺泡灌洗液中透明质酸、层粘连蛋白含量及骨组织中基质金属蛋白酶-1(MMP-1)mRNA含量的影响,利用熏烟并施以寒燥环境刺激的方法建立大鼠模型,使用酶联免疫吸附测定法(Enzyme-Linked Immunoadsordent Assay,ELISA)测其外周血血清中MMP-9和TNF-α以及肺泡灌洗液中透明质酸、层粘连蛋白含量,使用荧光定量PCR法检测骨组织中MMP-1 mRNA的表达。结果表明,大鼠血清中模型组血清MMP-9含量高于对照组(P<0.01),血清中TNF-α、肺泡灌洗液中透明质酸、层粘连蛋白含量模型组高于对照组。骨组织中MMP-1 mRNA表达模型组高于对照组(P<0.05)。熏烟复合模拟新疆的寒燥环境导致的机体血清中MMP-9和TNF-α,肺泡灌洗液中透明质酸、层粘连蛋白增多,骨组织中MMP-1 mRNA表达增高的现象,可能是熏烟复合寒燥环境扰乱机体内环境稳态的微观机制的部分表现。

Influence of Cigarette Smoking and Cold Dryness on Matrix Metalloproteinase and Airway Extracellular Matrix in Rat

To establish the model by cigarette smoking and stimulus of cold and dryness environment, the Enzyme-linked immunoadsordent assay method was used to measure MMP-9 and TNF-α content in rat's blood serum and HA, LN in BALF, fluorescence quantitative PCR to determine the expression of MMP-1mRNA in bone. The results were analyzed by using SPSS 11.5 statistical software to see the effects of cigarette smoking and cold dryness in Xinjiang' environment on matrix metalloproteinase -9 (MMP-9) and tumor necrosis factor-α (TNF-α) in rat's serum, HA, LN in BALF , and MMP-1 mRNA in rat's bone. The results show that MMP-9 level is significantly higher than that in the control group (P<0.01), between the two groups, TNF-α in serum, HA, LN in BALF, are not significantly different, but the model group's figures are larger. The expression of MMP-1 mRNA is higher in the cold and dry group than in the control group (P<0.05). So it may be concluded that cigarette smoking and cold dryness environment can stimulate the increase of MMP-9 and TNF-α in rat's serum, HA, LN in BALF and MMP-1 mRNA in rat's bone. And this may be part of the mechanism that cigarette smoking and cold dryness environment lead to disorders of the body.