| 海生红藻多管藻中的2种R-藻红蛋白 |
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| 引用本文： | 华萌萌,赵明日,付学军,龚雪琴,孙力.海生红藻多管藻中的2种R-藻红蛋白[J].烟台大学学报(自然科学与工程版),2011,24(3):201-208. |
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| 作者姓名： | 华萌萌 赵明日 付学军 龚雪琴 孙力 |
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| 作者单位： | 烟台大学化学生物理工学院,山东烟台,264005 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 以新鲜的海生红藻多管藻(Polysiphonia urceolata Grev)为材料,通过DEAE-52离子交换层析,Sephadex G-150和Sephacryl S-300凝胶过滤层析纯化制备了2种R-藻红蛋白(R-phycoerythrin,R-PE)PE272-1和PE377-2.两者有非常相似的光谱特性,...
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| 关 键 词： | 红藻 多管藻 藻胆体 R-藻红蛋白 |
Two R-phycoerythrins from a Marine Red Alga Polysiphonia urceolata Grev |
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| HUA Meng-meng,ZHAO Ming-ri,FU Xue-jun,GONG Xue-qin,SUN Li.Two R-phycoerythrins from a Marine Red Alga Polysiphonia urceolata Grev[J].Journal of Yantai University(Natural Science and Engineering edirion),2011,24(3):201-208. |
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| Authors: | HUA Meng-meng ZHAO Ming-ri FU Xue-jun GONG Xue-qin SUN Li |
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| Institution: | HUA Meng-meng,ZHAO Ming-ri,FU Xue-jun,GONG Xue-qin,SUN Li(School of Chemical and Biological Science and Engineering,Yantai University,Yantai 264005,China) |
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| Abstract: | Two R-phycoerythrins(R-PE),PE272-1 and PE377-2,are isolated and purified from the marine red alga Polysiphonia urceolata by the ion exchange chromatography on DEAE-52 and by the gel filtration chromatography on Sephadex G-150 and Sephacryl S-300.The two prepared R-PEs have very similar spectral properties and show single band both in native PAGE and in native isoelectric focusing(IEF).Isoelectric points(pIs) of PE272-1 and PE377-2 are 4.5 and 4.4,respectively.The polypeptide analysis by SDS-PAGE and denatur... |
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| Keywords: | red alga Polysiphonia urceolata Grev phycobilisome R-phycoerythrinEstablishment of Assay for Screening of Elastase Inhibitor and |
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