Chromosome G-banding in situ hybridization of RFLP marker umc58 linked with the gene hm1 dictatingHelminthosporium carbonum susceptibility1 in maize

The technique of simultaneous G-banding and in situ hybridization has been developed in plants for the first time. Using this technique, RFLP marker umc58 closely linked with the hm1 gene dictatingHelminthosporium carbonum susceptibility1 was localized onto 1L3 (chromosome 1, long arm, the third band from the centromere to the end of the arm), 5L5 and 9L5. The results demonstrated that umc58 was a triplicated sequence. It was deduced that umc58 probably was in a duplicated region that includes a part ofHelminthosporium carbonum susceptibility genes (hm1 and hm2), as the hybridization sites of umc58 in chromosomes 1 and 9 were those at which the genes localize. The techniques of simultaneous G-banding and ISH in plants are discussed.