| 菊各器官的组织培养 |
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| 引用本文： | 张嘉宝,蔡朝晖,付蕾,王台,陈明.菊各器官的组织培养[J].河南师范大学学报(自然科学版),1986(1). |
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| 作者姓名： | 张嘉宝 蔡朝晖 付蕾 王台 陈明 |
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| 作者单位： | 湖南师范大学1985届生物系，湖南师范大学1985届生物系，湖南师范大学1985届生物系，湖南师范大学1985届生物系 毕业生，毕业生，毕业生，毕业生 |
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| 摘 要： | 菊各器官的离体培养采用MS基本培养基。诱导培养基为MS培养基附加2,4—D1mg/1、6—BA2mg/1、NAAlmg/1或附加6—BA2mg/1、NAA0.5mg/1、LH500 mg/1;分化培养基为MS培养基附加NAA0.5mg/1,6—BA2mg/1,;生根培养基为MS培养基附加IBA2mg/1,将MS培养基中的大量元素减半,附加活性碳200mg/1,对菊花瓣愈伤组织的形成进行了比较实验。所有培养基的蔗糖浓度均为3%,pH5.8～6.0,琼脂为0.7～1.0%。经一段时间的培养,叶叶能再生出完整植株,并且移栽成功;叶柄及茎形成无根小苗;花瓣能再生出根。花序轴、花苞片均有愈伤组织形成;根末形成愈伤组织。
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THE TISSUE CUITURE OF EVERY ORGANS OF DENDRANTHEMA MORIFOLIUM(RAMAT)TZVEI |
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| Abstract: | Ms media was used for the tissue culture of explants of every organs ofDendranthema morifolium(Ramat)Tzvel.Induction media was Supplimentedwith 2,4—Dlmg/1、6-BA2mg/1,NAA lmg/1 or 6—BA2mg/1、NAA0.5mg/1、
LH500mg/1 on MS media;Differentiation media with NAA0.5mg,/1、6—BA2 mg/1;Rooten media with IBA 2mg/1,The compare teat of the callus form-ation of petal of Chrysanthemum has been conducted through reduction by ha-
lf of mass-element in MS media or/and Supplimented with active carbon 200mg/1,All media above were added with Sucrose with concentration of 3%,pH5.8～6.0,and with agar 0.7—1.0%,After a period of culture,regeneration
plantlets was obtained by culture of leaf,The plant of normal growth hasbeen gained after transplantation plantlets into Soil Free—root plantletswas obtained by culture of leafstalk and Stem.The root was differentiatedby culture of petal.The callus was obtained by culture of inflorescence andcalyx,no callus by culture of root. |
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