| 重组表达载体pGEM-VLA4的构建 |
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| 引用本文： | 安利峰,胜利,马力杨.重组表达载体pGEM-VLA4的构建[J].西北民族学院学报,2006,27(4):41-43. |
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| 作者姓名： | 安利峰 胜利 马力杨 |
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| 作者单位： | 西北民族大学医学院 甘肃兰州730030 |
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| 基金项目： | 西北民族大学中青年科技基金项目(D2005-010) |
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| 摘 要： | 从人外周血细胞中提取mRNA,用RT-PCR法扩增含BamH与HindⅢ酶切位点VLA4基因序列,运用分子生物学方法将该序列克隆到pGEM中,构建成VLA4基因序列的真核表达载体并进行鉴定分析.对重组载体用双酶切系统酶切后进行电泳并测序,结果证明目的基因克隆及连接方向正确,测序结果正确.通过实验,成功地构建了含VLA4基因的真核表达载体pGEM-VLA4.
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| 关 键 词： | 载体 VLA4 构建 |
| 文章编号： | 1009-2102(2006)04-0041-03 |
| 修稿时间： | 2006年10月20 |
Construction about Recombination Expression Vector of pGEM- VLA4 |
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| AN Li-feng,SHENG Li,MA Li-yang.Construction about Recombination Expression Vector of pGEM- VLA4[J].Journal of Northwest Minorities University(Natural Science ),2006,27(4):41-43. |
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| Authors: | AN Li-feng SHENG Li MA Li-yang |
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| Abstract: | Objective: to construct recombination expression vector of pGEM-VLA4.Method: The mRNA of VLA4 was extracted from human peripheral blood cells——VLA4 rearrangement gene containing BamH and HindⅢ endoenzyme sites were obtained by using RT-PCR method.Molecular biological method was used to clone the gene to pGEM.Results: The recombination expression vector were cut by the system of double enzyme cuting.Target gene cloning and linking direction were proved to be correct,and the result of sequence measurement was correct.Conclusion: This study successfully constructed recombination expression vector of pGEM-VLA4. |
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| Keywords: | vector VLA4 construction |
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