| 薄层层析—光度法对蜂集花粉中氨基酸的定性定量分析 |
| |
| 引用本文： | 林垚,梁丹,张永亮,金声,黄向红.薄层层析—光度法对蜂集花粉中氨基酸的定性定量分析[J].北京大学学报(自然科学版),1991(6). |
| |
| 作者姓名： | 林垚 梁丹 张永亮 金声 黄向红 |
| |
| 作者单位： | 北京大学化学系
(林垚，梁丹，张永亮，金声)，温州大学(黄向红) |
| |
| 摘 要： | 本文以党参、油菜、水烛及罂粟等4种植物的蜂集花粉进行醇—水提取,经离子交换柱后得到游离氨基酸样品,提取后的花粉残渣以6ml/l盐酸封管水解制得结合氨基酸样品。两种样品经硅胶薄层双向展开鉴定了上述花粉中游离、结合氨基酸的种类,并进行色斑提取,经光度分析测定出相应的含量。本法简便可靠,特别适合于含未知结构氨基酸样品的制备分离及分析。
|
| 关 键 词： | 薄层层析—光度法 花粉 氨基酸 |
TLC-spectrophotometry for Qualitative and Quantitative Analysis of Bee-collected Pollen |
| |
| LIN Yao,LIANG Dan,ZHANG Yongliang,JIN Sheng.TLC-spectrophotometry for Qualitative and Quantitative Analysis of Bee-collected Pollen[J].Acta Scientiarum Naturalium Universitatis Pekinensis,1991(6). |
| |
| Authors: | LIN Yao LIANG Dan ZHANG Yongliang JIN Sheng |
| |
| Abstract: | The samples of free amino acids were prepared from bee-collected pollens of codo-nopsis pilosula, brassica campestris, typha angustifilia and papaver, through extraction with ethanol-water solution and further purified by ion-exchange chromatographic columns. The pollen resides after extraction gave out the binding amino acids samples upon hydrolysis with 6mol/L HCI solution in sealed tubes. These two kinds of samples were developed two-dimensionally on silica-gel thin layers for the identification of both free and bonding amino acids. The relevant content of the identified amino acid was determined by the spectrophotometric analysis of the layer-spot extracts. Such methods are reliable, easy-operating and particularly fit for the preparative separation and analysis of the sample containing unknown amino acids. |
| |
| Keywords: | TLC-spectorphotometry Pollen Amino-acids |
| 本文献已被 CNKI 等数据库收录！ |
|
