| 罗丹明6G生物探针测定蛋白质 |
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| 引用本文： | 刘立明,宋功武,方光荣,李玲,王小妍.罗丹明6G生物探针测定蛋白质[J].湖北大学学报(自然科学版),2001,23(4):363-365. |
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| 作者姓名： | 刘立明 宋功武 方光荣 李玲 王小妍 |
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| 作者单位： | 湖北大学化学与材料科学学院，湖北武汉430062 |
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| 基金项目： | 中国分析测试基金资助课题 ( 99-11) |
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| 摘 要： | 以荧光染料单体和二聚体平衡转化为基础，提出了以罗丹明6G荧光染料为生物探针测定蛋白质的方法。罗丹明6G在十二烷基磺酸钠（SDS）的存在下形成二聚体，蛋白质的定量加入可以调节这一平衡，据此拟定测定蛋白质的分析方法，该法线性范围宽，灵敏度高，反应速度快，正确度高，抗干扰能力强，对样品的分析结果令人满意。
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| 关 键 词： | 荧光染料 罗丹明6G 表面活性剂 蛋白质 生物探针 |
| 文章编号： | 1000-2375(2001)04-0363-03 |
| 修稿时间： | 2001年5月8日 |
Fluorescent dye,Rhodamine 6G as biological probe for the determination of protein |
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| Liu Liming,Song Gongwu,Fang Guangrong,Li Ning,Wang Xiaoyan.Fluorescent dye,Rhodamine 6G as biological probe for the determination of protein[J].Journal of Hubei University(Natural Science Edition),2001,23(4):363-365. |
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| Authors: | Liu Liming Song Gongwu Fang Guangrong Li Ning Wang Xiaoyan |
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| Abstract: | Gived a novel method which Rhodamine 6G,a fluorescent dye was used as biological probe for the determination of protein.The method was based on the equilbrium shift between monomer and dimmer of fluorescent dyes.Rhodamine 6G was formed its dimmer in the presence of a cation surfactant.The equilibrium can be adjusted by the quantitation addition of protein.The significant features of the method were its high sensitivety and rapidity of reaction,wide range of linearity,high accuracy and selectivity. |
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| Keywords: | fluorescent dyes Rhodamine 6G cationic surfactant protein |
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