茜素紫光度法检测H2 O2／CO2+产生的羟自由基

研究了用茜素紫的氧化来显色测定Co2+-H2O2体系产生羟自由基(·OH)的方法，提出CO2+-H2O2-茜素紫分析新体系并用于羟自由基(·OH)的测定．本方法利用Co2+与H2O2反应，类似Fenton试剂产生羟自由基(·OH)，并加入茜素紫显色剂，使茜素紫的颜色发生变化，采用紫外．可见分光光度计测定其△A值的变化，可间接测定羟自由基的含量，且灵敏度高．研究结果表明：在最大吸收波长560nm处，茜素紫的吸光度与·OH产生量成良好的线性关系；此外，抗氧化荆药物甘露醇、苯甲酸、硫脲与羟自由基清除率具有明显的量效关系，方法稳定性好。操作简便，测定快速，是一种简便的筛选抗羟自由基的清除剂和有效抗氧化剂药物的方法

Assay of hydroxyl radical produced by CO2 +/H2O2 reaction using alizarin violet spectrophotometry

A method of determining hydroxyl radical produced by Co2 + -H2 O2 system by chromogenic reaction of oxida-tion of alizarin violet has been introduced in this paper. The new analytical system of Co2 + -H2 O2-alizafin violet has beenfirst put forward and applied to determine Hydroxyl radicals. In this method, hydroxyl radicals are produced utilizing thereaction of Co2+ -H2 02 resembling the Fenton reaction, and at the same time, the chromogenic reagent of alizarin violet isadded to change the color of alizarin violet. The amount of hydroxyl radicals can be calculated indirectly by determiningthe variety of value of △A by UV absorption spectrum. The sensitivity of method is wholly higher than the classic ways ofFenton reaction. The optimum condition has been found by researching into the condition of determination. According tothe result, it is found that an apparent Iinear relation exists in the anti-oxidation radicals such as ascorbic acid, benzoicacid and hydroxyl radical. The results show that in the maximum wave of 560 nm, there is an excellent linear relationshipexisting between the absorption of alizarin violet and the production of · OH. This method has a merit of good stability,handy operation and quick determination so that it can be easily adopted by general laboratory and applied to filtrate anti-oxidation radicals as a handy elihmination reagent. It is also an effective method to filtrate medicament of anti-oxidation