| 果蝇醇脱氢酶S—139定点突变后活性的变化 |
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| 引用本文： | 童耕雷,林志新.果蝇醇脱氢酶S—139定点突变后活性的变化[J].上海交通大学学报,1997,31(4):77-81. |
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| 作者姓名： | 童耕雷 林志新 |
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| 摘 要： | 经酶动力学分析，丝氨酸－１３９的突变体Ｓ１３９Ａ使ＤｍＡＤＨ完全失去催化活性。Ｓ１３９Ｃ和Ｓ１３９Ｔ使ＤｍＡＤＨ的催化能力分别下降为野生型的１４．３％和４１．６％以及４７％和７６％，而且Ｋｃａｔ／Ｋｍ（Ａｌｃ）和ＣＡＴ（Ｋｍ（ＮＡＤ）差异极为显著；对于乙醇，突变体相对于野生型变化不大；而对于ＮＡＤ，突变体相对于野生型下降到原来的１０％以上。
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| 关 键 词： | 果蝇 醇脱氢酶 人工定点突变 突变体 酶动力学 |
Change in Activity of Drosophila Melanogaster S 139 after Site Directed Mutagenesis |
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| Tong Genglei,Lin Zhixin.Change in Activity of Drosophila Melanogaster S 139 after Site Directed Mutagenesis[J].Journal of Shanghai Jiaotong University,1997,31(4):77-81. |
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| Authors: | Tong Genglei Lin Zhixin |
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| Institution: | Department of Biological Science and Technology |
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| Abstract: | According to the analysis of enzyme dynamics, mutating ser 139 to alanine (S139A) virtually inactivats DmADH, however, substitutions of cysteine (S139C) and Threonine (S139T) at this position cause decrease to 14.3% and 41.6% (for propan 2 ol) 47% and 76% (for ethanol) respectively. The difference between K cat / K m(Alc) and K cat / K m(NAD) is distinct: for ethanol the substitutions (S139C and S139T) cause only a little decrease (in contrast to the wild type) but for NAD more than 90% decrease. |
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| Keywords: | drosophila melanogaster alcohol dehydrogenase site directed mutagenesis S 139 A |
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