古巴芦荟的组织培养

试验结果表明：古巴芦荟（ＡｌｏｅｖｅｒａＬ．）对蔗糖浓度适应范围较大（２％～５％），其中以３％的浓度最适宜．在侧芽诱导中以ＭＳ基本培养基较合适．激素对侧芽的诱导作用符合细胞分裂素／生长素的器官发生调节模式，其中以ＭＳ＋ＢＡ３—５ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ对芽的诱导最有效，而ＫＣ＋ＩＢＡ２—３ｍｇ／Ｌ对根的诱导最佳，生根率达１００％．在生根培养阶段加入１－３ｍｇ／Ｌ的活性碳是必要的．试管苗移栽的最佳基质是河沙，成活率达９０．２％．

Tissue Culture of Aloe vera L.

Experimental results demonstrate that cuba aloe (Aloe vera L. ) can acclimatizes to wide range of sucrose concentration and 3% sucrose concentration is optimum. For inducing cateral buds, MS medimm is quite suitable. The inducing effects of plant growth regulators on the development of shoots and roots are in accordance with the organ developing and regulating caused by cytokinin/auxin. The medium of MS+3-5 mg/L BA+0.2 mg/L NAA is optimum for shoot inducing and the medium of KC + 2 -3 mg/L IBA is optimum for root inducing. Besides, the rooting rate can be as high as 100%. It is necessary to add 1-3 gram AC/L to the medium in rooting culture period. The best matrix for plantlet transfer cultivating is riversand. Surviving rate of plantlet can reach 90. 2%.