流式荧光微球技术在实验动物质量控制中的应用

摘要: ELISA 和IFA 是实验室主要的血清学检测方法，在过去的数十年中得到广泛的应用。但其存在着操作繁琐、 耗时长的问题。美国Luminex 公司开发的xMAP 是一种快速、高通道的技术平台，可在同一反应孔内同时检测上 百种抗原、抗体、核苷酸片段、受体/配体等生物分子，在医学界已广泛应用。MFIATM 是美国Charles River Laboratories 应用xMAP 平台开发的应用于实验动物质量控制的多通路、高效率的抗体检测技术，该技术的应用大 大改善了实验动物质量控制的水平。

Application of Fluorescent Microsphere Technology on Laboratory Animal Quality Control

For the past several decades,ELISA and IFA have been used as the primary assays for viral or bacterial antibody detection.However,these traditional methods are either time-consuming or labor-intensive.Microsphere-based suspension array technologies,such as the Luminex xMAP system,offer a new platform for high-throughput biomolecule detection which has been used in a variety of research fields.Using LuminexCorporation’s xMAP technology,the Charles River Laboratories has developed Multiplexed Fluorometric ImmunoAssayTM(MFIATM) profiles for sero-surveillance of various laboratory animal species,which has promoted the level of laboratory animal quality control significantly.