BALB/c与C57BL/6小鼠互为供受体心脏移植急性排斥模型的对比

目的建立并比较BALB/c与C57BL/6小鼠互为供受体心脏移植急性排斥模型。方法采用BALB/c与C57BL/6小鼠互为供受体建立心脏移植模型,术后评估免疫排斥情况、并发症、观察移植心脏存活时间并做病理检查。结果手术成功率为94.7%。A组(BALB/c→C57BL/6)与B组(C57BL/6→BALB/c)的生存时间无统计学差异。两组均可见典型急性排斥病理改变,两组间无明显统计学差异。两组移植心脏的心功能情况无明显差异。结论近交系BALB/c和C57BL/6基因小鼠遗传稳定,可以建立稳定的急性排斥反应模型。C57BL/6适合作为受体,移植时间明显缩短,造模成功率较高。

Comparison of Donor-recipient in BALB/c and C57BL/6 Mice on Acute Cardiac Allograft Rejection Model

Objective To establish and compare of donor-recipient in BALB/c and C57BL/6 mice on acute cardiac allograft rejection model.Methods Making BALB/c and C57BL/6 mice with donor-recipient on cardiac transplantation model and assessment of the immune rejection situation and complications,the survival time was observed and cardiac allograft was harvested for histopathological evaluation.Result Success rate was 94.7%.A group（BALB/c → C57BL / 6） and B group（C57BL/6 → BALB/c） have no significant difference in survival time.Two groups have typical pathological changes of acute rejection,and have no significant statistical difference.Two groups of heart function after heart transplantation also have no significant difference.Conclusion Inbred BALB/c and C57BL/6 mice have genetically stable characteristic,different donor-recipient combinations between BALB/c and C57BL/6 inbred mice are suitable for acute rejection model.C57BL / 6 as recipient are rational choice because the transplantation time was significantly shorter and the higher the success rate in model.