Research at channel level on the effect of LaCl3 on Radish (Raphanus satirus L.) vacuolar membrane |
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Authors: | Yanfang Shang Pin Yang Zhenming Pei |
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Institution: | 1. Institute of Molecular Science, Shanxi University, 030006, Taiyuan, China 2. Department of Biology, Duke University, 27708, Durham, NC, USA
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Abstract: | We recorded slow vacuolar (SV-type) channel currents of Radish vacuoles successfully for the first time by using the whole-vacuolar patch-clamp recording mode. SVtrye currents would increase and threshold potentials of activation would shift towards more negative values with the increase of concentrations of cytosolic Ca2+. When 2.5 mmol/L LaCl3 and 4 mmol/L EGTA were added to bath solutions,SV-type currents were suppressed remarkably. Then adding LaCl3 with different concentrations to pipette solutions, we found that LaCl3 with higher concentrations (> 4 × 10-7 mol/L)had a strong inhibitory effect on SV-type currents, while LaCl3 with lower concentrations (≤4 × 10-7 mol/L) promoted channel currents. This promoting effect provides an important basis at channel level for researching further the effects of rare earth on physiological activities of plants and the production-increase effects of rare earth fertilizers on crops.`` |
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Keywords: | whole-vacuolar patch-clamp recording SV-type channel current cytosolic Ca2+ La3+ |
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