| R—藻红蛋白色基多肽对肿瘤细胞光动力杀伤作用的实验研究 |
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| 引用本文： | 黄蓓,李振刚,王广策,刘基东,孙海宝,曾呈奎.R—藻红蛋白色基多肽对肿瘤细胞光动力杀伤作用的实验研究[J].中国科学技术大学学报,2001,31(2):241-246. |
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| 作者姓名： | 黄蓓 李振刚 王广策 刘基东 孙海宝 曾呈奎 |
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| 作者单位： | 1. 中国科学技术大学生命科学院,
2. 中国科学院青岛海洋研究所, |
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| 基金项目： | 国家海洋863基金支持项目(819-Q12) |
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| 摘 要： | 首次用胃酶降解及CM Sephedex C-50柱层析法获得了6种藻红蛋白的色基多肽，并用藻红蛋白及其色基多肽对两种肿瘤细胞作体外激光疗法增敏作用实验，S180小鼠腹水癌细胞株培养后分别用浓度为10，25，50，100ug/mL的藻红蛋白及其色基多肽处理，经波长为488nm的氩离子激光辐照（照射剂量为28．8J/cm2)，用MTT法检测，其细胞的生存率最佳效果分别棕达45％及16％，显示出良好的剂量效应，在激光处理藻红蛋白及其色基多肽对HL60人白血病细胞所做的细胞生长曲线图的比较中可看出，色基多肽的激光增敏作用要优于藻红蛋白多聚体。
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| 关 键 词： | 藻红蛋白 色基多肽 光动力学疗法 肿瘤 治疗 |
| 文章编号： | 0253-2778(2001)02-0241-06 |
The Experimental Research
of R-Phycoerythrin and Its Chromophore Polypeptides in the Photodynamic Therapy of Cancer |
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| HUANG Bei ,LI Zhen-gang ,WANG Guang-Ce ,LIU Ji-dong ,ZENG Chen-kui.The Experimental Research
of R-Phycoerythrin and Its Chromophore Polypeptides in the Photodynamic Therapy of Cancer[J].Journal of University of Science and Technology of China,2001,31(2):241-246. |
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| Authors: | HUANG Bei LI Zhen-gang WANG Guang-Ce LIU Ji-dong ZENG Chen-kui |
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| Institution: | HUANG Bei 1,LI Zhen-gang 1,WANG Guang-Ce 2,LIU Ji-dong 2,ZENG Chen-kui 2 |
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| Abstract: | It is the first time that six kinds of Chromophore Polypeptides of R-Phycoerythrin (R-PE)
were obtained from polysiphona urceolata by pepsin and CM-Sephedex C50 . In order to compare the
differetiation between R-PE and its Chromophore Polypeptides, two kinds of tumor cell lines were used
for an the experiment of photodynamic therapy (PDT) in vitro. S180 cell line was first treated with dif
ferent concentrations of R-PE and its Chromophore Polypeptides , and then irradiated with Ar-iron laser
( 488nm, 28.8 J/cm2). The survival rate was measured by MIT method. Moreover, the growth rate
of HL60 cells , which was treated with R-PE and its Chromophore Polypeptides. The results show that
the PDT effect of Chromophore Polypeptides is better than R-PE. |
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| Keywords: | R-Phycoerythrin chromophore polypeptides photodynamic therapy |
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