| 新型明胶纳米纤维毡的制备及其细胞培养 |
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| 引用本文： | 左璞晶,黄雅钦,张慎,陈国强,邓旭亮,杨小平.新型明胶纳米纤维毡的制备及其细胞培养[J].北京化工大学学报(自然科学版),2007,34(6):625-628. |
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| 作者姓名： | 左璞晶 黄雅钦 张慎 陈国强 邓旭亮 杨小平 |
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| 作者单位： | 1.北京化工大学材料科学与工程学院,北京,100029;2. 清华大学第二附属医院,北京 100084;3.北京大学口腔医学院,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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国家自然科学基金
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清华大学裕元医学科学基金 |
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| 摘 要： | 以水为溶剂通过静电纺丝法制备出了新型明胶纳米纤维。结果表明，当明胶的质量分数为33%和体系温度为40℃时，所得明胶纳米纤维毡具有均匀多孔的微观形貌，纤维直径分布在120～210nm。用质量分数为1.5%的1-(3-二甲氨基丙基)-3-乙基碳二亚胺（EDC）/N-羟基琥珀酰亚胺（NHS）的乙醇/水溶液对纤维毡进行交联，所得明胶纳米纤维毡中纤维的形态得到较好的保持，且其焓值降低，耐热性和力学性能均有所提高。将制备的明胶纳米纤维毡进行牙周基质细胞（PDLCs）培养实验，初步的结果表明PDLCs能够在该纳米纤维毡上粘附、伸展和繁殖。
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| 关 键 词： | 静电纺丝 明胶 纳米纤维 静电纺丝 明胶 纳米纤维 |
| 收稿时间： | 2007-03-19 |
| 修稿时间： | 2007年3月19日 |
Fabrication of a new type of gelatin nano-fiber and in-situ cell proliferation studies |
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| ZUO PuJing,HUANG YaQin,ZHANG Shen,CHEN GuoQiang,DENG XuLiang,YANG XiaoPing.Fabrication of a new type of gelatin nano-fiber and in-situ cell proliferation studies[J].Journal of Beijing University of Chemical Technology,2007,34(6):625-628. |
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| Authors: | ZUO PuJing HUANG YaQin ZHANG Shen CHEN GuoQiang DENG XuLiang YANG XiaoPing |
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| Institution: | 1. College of Materials Science and Technology, Beijing University of Chemical Technology, Beijing 100029; 2. Second Hospital of Tsinghua University, Beijing 100084; 3. Peking University School and Hospital of Stomatology, Beijing 100081, China |
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| Abstract: | A new type of gelatin nanofiber has been fabricated by electrospinning using water as the solvent. By using a 33% aqueous solution of gelatin at 40℃, gelatin anofiber mats having a uniform porous structure with fiber diameters in the range 120-210 nm were formed. After crosslinking with an aqueous ethanol solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide sodium salt (EDC/NHS), the morphology of the fibers in the gelatin nanofiber mats was retained. Crosslinking, led to a decrease in enthalpy but enhanced thermal resistance properties and mechanical strength.Proliferation experiments with periodontal ligament cells (PDLCs) indicated that the cells effectively adhered to, and proliferated on, the nanofiber mats, indicating that the material has considerable potential for use in guided tissue regeneration (GTR) in stomatology. |
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| Keywords: | electrospinning gelatin nanofiber |
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