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| 甜菜硝酸还原酶活性分析及基因片段的克隆 | |
| 引用本文： | 张杰,吴迪,彭胜民,姜月,刘岩,马凤鸣.甜菜硝酸还原酶活性分析及基因片段的克隆[J].哈尔滨商业大学学报(自然科学版),2008,24(5):610-613. |
| 作者姓名： | 张杰 吴迪 彭胜民 姜月 刘岩 马凤鸣 |
| 作者单位： | 1. 东北农业大学,农学院,哈尔滨,150030;东北林业大学,生命科学学院,哈尔滨,150040 2. 东北林业大学,生命科学学院,哈尔滨,150040 3. 东北农业大学,农学院,哈尔滨,150030 |
| 基金项目： | 国家自然科学基金 ， 东北林业大学校科研和教改项目 |
| 摘 要： | 为了明确缺氮条件下甜菜NR的活性，用活体测定法检测了经缺氮胁迫不同时间后甜菜叶中NR活性．结果表明，随着缺氮处理时间的延长，甜菜叶中的NR活性逐渐降低，在缺氯处理1h后，其活性下降较快．用50mmol／1．KNO3溶液处理的甜菜幼苗总RNA，通过RT—PCR分离得到了硝酸还原酶基因片段，长度为471bp，Blast分析表明，其与Genbank中硝酸还原酶基因部分序列具有高度同源性． |
| 关 键 词： | 甜菜 硝酸还原酶活性 基因克隆 RT—PCR |
Analyzing of nitrate reductase and cloning gene fragment sequence of sugar beet(Beta vulgaris L.) |
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| ZHANG Jie,WU Di,PENG Sheng-min,JIANG Yue,LIU Yan,MA Feng-ming.Analyzing of nitrate reductase and cloning gene fragment sequence of sugar beet(Beta vulgaris L.)[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2008,24(5):610-613. | |
| Authors: | ZHANG Jie WU Di PENG Sheng-min JIANG Yue LIU Yan MA Feng-ming |
| Institution: | ZHANG Jie1,2,WU Di2,PENG Sheng-min1,JIANG Yue2,LIU Yan 2,MA Feng-ming1 (1.School of Agronomy,Northeast Agricultural University,Harbin 150030,China,2.School of Life Sciences,Northeast Forestry University,Harbin 150040,China) |
| Abstract: | The experiments are conducted to investigate the activity of nitrate reductase in sugar beet(Beta vulgaris L.) under different time of N-absent treatment.The results show that the activity of NR rapidly decrease in the first 1 h and gradually decrease when the time of N-absent treatment is prolonged.The homolog cDNA with 471 base pairs of nitrate reductase gene is obtained by RT-PCR from total RNA using Beta vulgaris treated by 50 mmol/L KNO3 as material.Blast analysis showed high identity to part sequence ... |
| Keywords: | RT-PCR |
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