| 粉纹夜蛾5BI细胞的悬浮培养 |
| |
| 引用本文： | 彭建新,潭文松.粉纹夜蛾5BI细胞的悬浮培养[J].华中师范大学学报(自然科学版),1996,30(4):472-475. |
| |
| 作者姓名： | 彭建新 潭文松 |
| |
| 作者单位： | [1]华中师范大学昆虫学研究所 [2]华东理工大学生化工程研究所 |
| |
| 摘 要： | 报道了粉纹夜蛾５ＢＩ细胞在转瓶系统悬浮培养及血清浓度，细胞接种浓度，非离子表面活性剂Ｐｌｕｒｎｉｃ－Ｆ－６８等对细胞生长的影响与作用，实验结果显示：５ＢＩ细胞能够搅拌条件下，在含５％血清培养基中正常生长增殖，当血清浓度降至２％时。细胞生长速率产生较明显变化，ＰｌｕｒｏｎｉｃＦ－６８对细胞生长有显著的保护作用，５ＢＩ细胞适宜于悬浮培养和进一步放大培养。
|
| 关 键 词： | 粉纹夜蛾 5BI细胞 悬浮培养 病毒杀虫剂 |
THE SUSPENSION CULTURE OF 5BI CELL LINE FROM THICHOPUSIA NI BY SPINNER FLASK |
| |
| Peng Jianxin,Yang Hong,Hong Huazhu.THE SUSPENSION CULTURE OF 5BI CELL LINE FROM THICHOPUSIA NI BY SPINNER FLASK[J].Journal of Central China Normal University(Natural Sciences),1996,30(4):472-475. |
| |
| Authors: | Peng Jianxin Yang Hong Hong Huazhu |
| |
| Abstract: | The suspesion culture of 5BI cell line from Thichoplusia ni and culture conditions effcted on cell growth including serum concentration, seeding density and nonionic surfactant pluronic F 68 were studied in this paper. It was demonstrated that 5BI cell could normally grow and propagate under agitating condifion. Cell growth rate decreased as serum concentration was reduced to 2%. pluronic F 68 was responsible for protection of 5BI insect cells from advers effects of agitating. |
| |
| Keywords: | hichoplusia ni 5BI cell line from Trichoplusia ni suspension culture |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《华中师范大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《华中师范大学学报(自然科学版)》下载免费的PDF全文 |
