高效Ni 2+ 吸附菌株分离鉴定及特性研究

采用浓度梯度筛选的方法,从活性污泥中筛选到一株能耐受并吸附Ni 2+的菌株,命名为Ni-1.采用单因素法对菌株Ni-1的最适培养条件进行了考察,确定培养条件为t=30℃,pH=7.0,1.0%的NaCl.考察了菌株Ni-1对Cu2+和Ni 2+的吸附性能,并用表面响应法优化了吸附条件.菌株Ni-1吸附Cu2+的最优条件为Cu2+100mg/L、投加菌量(湿重)0.85g、溶液pH=6.0,在此条件下对Cu2+的最大吸附率为87.13%.菌株Ni-1吸附Ni 2+的最优条件为Ni 2+50mg/L、投加菌量(湿重)0.85g、溶液pH=6.0,在此条件下对Ni 2+的最大吸附率为84.32%.实验结果表明该细菌对较低浓度的Cu2+和Ni 2+有较好的吸附性能.

Isolation, identification and characterization of an efficient nickel accumulating strain

A strain capable of tolerating and adsorbing nickel,which was named Ni-1,was isolated from activated sludge by concentration gradient screening technique.According to the single-factor experiments,the optimum growth conditions are obtained as follows:temperature 30 ℃,pH=7.0,sodium chloride concentration 1.0%.Subsequently,the adsorption capability of strain Ni-1 for Cu2+ and Ni2+ was investigated,and the absorption conditions were optimized using response surface methodology(RSM).The optimal conditions for Cu2+ absorption by strain Ni-1 are as follows: 100 mg/L Cu2+,0.85 g strain Ni-1(wet weight),and pH=6.0.Under the optimized conditions,the absorption rate of Cu2+ can achieve 87.13%.The optimal conditions for Ni2+ absorption by strain Ni-1 are as follows:50 mg/L Ni2+,0.85 g strain Ni-1(wet weight),and pH=6.0.Under the optimized conditions,the absorption rate of Ni2+ can achieve 84.32%.Experimental results indicate that strain Ni-1 has good adsorption capacity for Cu2+ and Ni2+ at low concentrations.