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| Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice （Oryza sativa L.） | |
| 引用本文： | GUAN HuaZhong,DUAN YuanLin,LIU HuaQing,CHEN ZhiWei,ZHUO Ming,ZHUANG LiJun,QI WenMing,PAN RunSen,MAO DaMei,ZHOU YuanChang,WANG Feng,WU WeiRen. Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice （Oryza sativa L.）[J]. 科学通报(英文版), 2011, 56(14): 1476-1480. DOI: 10.1007/s11434-011-4552-9 |
| 作者姓名： | GUAN HuaZhong DUAN YuanLin LIU HuaQing CHEN ZhiWei ZHUO Ming ZHUANG LiJun QI WenMing PAN RunSen MAO DaMei ZHOU YuanChang WANG Feng WU WeiRen |
| 作者单位： | [1]Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agricultural ＆ Forestry University Fuzhou 350002, China [2]Fujian Provincial Key Laboratory of Marker-Assisted Breeding of Rice, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3]Fujian Agricultural Science Research Institute, Fuzhou 350002, China |
| 基金项目： | We thank Lin Lihui, Lan Tao, Zhao JizhL Zhang Xuzhang, Yang Fan, Guan Zhiqin, Tang Weiqi, Zhang Shiyuan, Chen Liping, Lin Yanqiu and Huang Ding of the College of Crop Science, Fujian Agriculture and Forestry University, for their assistance in the population construction. This work was supported by the National Transgenic Projects of China （2009ZX08009-109B）, Natural Science Foundation of Fujian Province （2010J01077）, the National High Technology Research and Development Program of China （2006AA10Z128） and New Century Excellent Talents in University of Fujian Province （KY0010057）. |
| 摘 要： | The phenomenon of panicle enclosure in rice is mainly caused by the shortening of uppermost internode.Elucidating the molecular mechanism of panicle enclosure will be helpful for solving the problem of panicle enclosure in male sterile lines and creating new germplasms in rice.We acquired a monogenic recessive enclosed panicle mutant,named as esp2 (enclosed shorter panicle 2),from the tissue culture progeny of indica rice cultivar Minghui-86.In the mutant,panicles were entirely enclosed by flag leaf sheaths and the uppermost internode was almost completely degenerated,but the other internodes did not have obvious changes in length.Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene,which could be steadily inherited and was not affected by genetic background.Apparently,ESP2 is a key gene for the development of uppermost internode in rice.Using an F 2 population of a cross between esp2 and a japonica rice cultivar Xiushui-13 as well as SSR and InDel markers,we fine mapped ESP2 to a 14-kb region on the end of the short arm of chromosome 1.According to the rice genome sequence annotation,only one intact gene exists in this region,namely,a putative phosphatidylserine synthase gene.Sequencing analysis on the mutant and the wild type indicated that this gene was inserted by a 5287-bp retrotransposon sequence.Hence,we took this gene as a candidate of ESP2.The results of this study will facilitate the cloning and functional analysis of ESP2 gene. |
| 关 键 词： | rice enclosed panicle mutant ESP2 gene fine mapping |
Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice (Oryza sativa L.) |
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| HuaZhong Guan,YuanLin Duan,HuaQing Liu,ZhiWei Chen,Ming Zhuo,LiJun Zhuang,WenMing Qi,RunSen Pan,DaMei Mao,YuanChang Zhou,Feng Wang,WeiRen Wu. Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice (Oryza sativa L.)[J]. Chinese science bulletin, 2011, 56(14): 1476-1480. DOI: 10.1007/s11434-011-4552-9 | |
| Authors: | HuaZhong Guan YuanLin Duan HuaQing Liu ZhiWei Chen Ming Zhuo LiJun Zhuang WenMing Qi RunSen Pan DaMei Mao YuanChang Zhou Feng Wang WeiRen Wu |
| Affiliation: | 1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agricultural & Forestry University, Fuzhou, 350002, China 2. Fujian Provincial Key Laboratory of Marker-Assisted Breeding of Rice, Fujian Agriculture and Forestry University, Fuzhou, 350002, China 3. Fujian Agricultural Science Research Institute, Fuzhou, 350002, China |
| Abstract: | The phenomenon of panicle enclosure in rice is mainly caused by the shortening of uppermost internode. Elucidating the molecular mechanism of panicle enclosure will be helpful for solving the problem of panicle enclosure in male sterile lines and creating new germplasms in rice. We acquired a monogenic recessive enclosed panicle mutant, named as esp2 (enclosed shorter panicle 2), from the tissue culture progeny of indica rice cultivar Minghui-86. In the mutant, panicles were entirely enclosed by flag leaf sheaths and the uppermost internode was almost completely degenerated, but the other internodes did not have obvious changes in length. Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene, which could be steadily inherited and was not affected by genetic background. Apparently, ESP2 is a key gene for the development of uppermost internode in rice. Using an F2 population of a cross between esp2 and a japonica rice cultivar Xiushui-13 as well as SSR and InDel markers, we fine mapped ESP2 to a 14-kb region on the end of the short arm of chromosome 1. According to the rice genome sequence annotation, only one intact gene exists in this region, namely, a putative phosphatidylserine synthase gene. Sequencing analysis on the mutant and the wild type indicated that this gene was inserted by a 5287-bp retrotransposon sequence. Hence, we took this gene as a candidate of ESP2. The results of this study will facilitate the cloning and functional analysis of ESP2 gene. |
| Keywords: | rice enclosed panicle mutant ESP2 gene fine mapping |
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