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Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR |
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| Authors: | An Yan GuoHui Ding ZhenFeng Zhou Hui Dong YaKun Zhang Lei Zhu YunGang He GuoQing Zhang YiXue Li Bing Sun Zhong Huang Ke Lan Li Jin HongYan Wang XiaoNing Wang Zhong Yang Yang Zhong JianXin Dai YaJun Guo Hao Wang XiaoYan Che Fan Wu ZhenGan Yuan Xi Zhang ZhiWei Cao XiaoNong Zhou JiaHai Zhou ZhiYong Ma GuangZhi Tong GuoPing Zhao WeiRong Jin Hui Xiong |
| Affiliation: | 1. Shanghai-MOST Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China 2. Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (Lab of Computational Genomics, CAS-MPG Partner Institute of Computational Biology Center of Bioinformatics, Key Lab of System Biology; Lab of Synthetic Biology, Institute of Plant Physiology & Ecology; Institute Pasteur), Shanghai, 200031, China 3. National Engineering Research Center for Biochip Technology, Shanghai Biochip Co. Ltd, Shanghai, 201203, China 4. Shanghai Center for Bioinformation Technology, Shanghai, 200235, China 5. School of Life Sciences, Fudan University, Shanghai, 200433, China 6. School of Life Sciences and Technology, South China University of Technology, Guangzhou, 510641, China 7. Cancer Institute, Second Military Medical University, Shanghai, 200433, China 8. National Engineering Research Center for Antibody Drugs, Shanghai, 201203, China 9. Zhujiang Hospical, Southern Medical University, Guangzhou, 510282, China 10. Shanghai Municipal Center for Disease Control & Prevention, Shanghai, 200336, China 11. School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China 12. National Institute for Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, China 13. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, China 14. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China |
| Abstract: | The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin (HA) of several subtypes (including H1, H2, H3, H5, H7 and H9) were designed. Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential. Contribute equally to this work. |
| Keywords: | influenza A virus HA gene H1 and H3 subtypes specific amplification by RT-PCT DNA sequencing |
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