| 钙调磷酸酶B亚基钙结合位点的突变对B亚基结构和功能的影响 |
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| 引用本文： | 尹燕霞,佟丽,黄建华,姜国华.钙调磷酸酶B亚基钙结合位点的突变对B亚基结构和功能的影响[J].北京师范大学学报(自然科学版),2021,57(2):205-212. |
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| 作者姓名： | 尹燕霞 佟丽 黄建华 姜国华 |
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| 作者单位： | 1.北京师范大学生命科学学院，北京市基因工程药物及生物技术重点实验室，100875，北京 |
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| 基金项目： | 国家自然科学基金资助项目（30770478） |
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| 摘 要： | 应用远紫外CD光谱、紫外差光谱、内源荧光光谱以及ANS荧光光谱，探究了钙调磷酸酶B亚基钙结合位点的突变对B亚基结构和功能的影响．结果显示:钙结合位点突变体Y105W激活CNA的能力强于CNB；而E110Q活化CNA的能力弱于CNB；Y105W和E110Q与Ca2+亲和性低于CNB；CNB和它的突变体二级结构趋于一致，但三级结构存在明显差异，这种差异可能是它们功能差异的结构基础．
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| 关 键 词： | 钙调磷酸酶 Ca2+ 磷酸酶活性 三级结构 钙结合位点 |
| 收稿时间： | 2020-05-18 |
Structure and function of calcineurin B subunit:mutation in the calcium binding site |
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| YIN Yanxia,TONG Li,HUANG Jianhua,JIANG Guohua.Structure and function of calcineurin B subunit:mutation in the calcium binding site[J].Journal of Beijing Normal University(Natural Science),2021,57(2):205-212. |
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| Authors: | YIN Yanxia TONG Li HUANG Jianhua JIANG Guohua |
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| Institution: | 1.College of Life Sciences, Beijing Key Laboratory of Genetic Engineering Drugs and Biotechnology, Beijing Normal University, 100875, Beijing, China2.Analysis and Testing Center, Beijing Normal University, 100875, Beijing, China |
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| Abstract: | Effect of mutations in the calcium binding site on calcineurin B subunit structure and function was investigated, by far ultraviolet CD spectrum, ultraviolet difference spectrum, endogenous fluorescence spectrum and ANS fluorescence spectrum．It was found that calcium binding site mutant CNB-Y105W was stronger than wild type CNB to activate CNA, but CNB-E110Q was weaker．The affinity of CNB-Y105W and CNB-E110Q for Ca2+ was both found to be lower than CNB．The secondary structures were found to be similar among CNB and the 2 mutants, but their tertiary structures showed significant differences, consistent with their differences in protein activity． |
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