混合菌构建及其对硝基苯直接好氧生物降解

选取本实验室分离的3株硝基苯高效降解菌———Rhodotorula mucilaginosa Z1、Streptomyces albidoflavusZ2和Micrococcus luteusZ3,将其混合并进行正交实验,得出Z1、Z2、Z3的最佳配比为1∶3∶3,在此基础上构建了混合菌.与单菌相比,混合菌能够在较为苛刻的环境条件下降解硝基苯,并具有较宽的底物范围.同时结合实际硝基苯工业废水特点,考察了混合菌在高盐度下以及苯酚或苯胺和硝基苯共存时对硝基苯的降解.结果表明:混合菌具备较强的耐盐能力,可在5%的高盐(NaCl)条件下有效降解硝基苯;当苯酚或苯胺和硝基苯(200mg/L)共存,初始浓度分别为100和50mg/L时,混合菌对硝基苯的降解不受影响.

Construction of microbial consortium and its directly aerobic biodegradation of nitrobenzene

Three effective nitrobenzene degrading strains, Rhodotorula mucilaginosa Z1, Streptomyces albidoflavus Z2 and Micrococcus luteus Z3 were isolated and identified. A defined microbial consortium was constructed through orthogonal experiments by using Z1, Z2 and Z3. The optimal ratio of Z1 to Z2 to Z3 is 1:3 :3. Compared with pure strains, the consortium can degrade nitrobenzene under severe conditions and has a wider substrates range. Considering the characteristics of actual nitrobenzene wastewater, nitrobenzene degradation was investigated by the consortium under high salinity, as well as phenol or aniline co-existence with nitrobenzene. The consortium can degrade nitrobenzene effectively in high salinity of 5% (NaCl). The degradation of nitrobenzene (200 mg/L) by the consortium is not influenced in the presence of 100 mg/L phenol or 50 mg/L aniline.