| 反胶束固定化醇脱氢酶的制备 |
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| 引用本文： | 何进星,柳畅先.反胶束固定化醇脱氢酶的制备[J].中南民族大学学报(自然科学版),2008,27(4). |
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| 作者姓名： | 何进星 柳畅先 |
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| 作者单位： | 中南民族大学,化学与材料科学学院分析化学国家民委重点实验室,武汉,430074 |
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| 摘 要： | 报道了用反胶束体系固定化醇脱氢酶(ADH)的研究.在此体系中,以十六烷基三甲基溴化铵(CTAB)作为表面活性剂,辛烷和己醇作为溶剂及助溶剂.考察了ADH固定化的影响因素,确立了最佳固定化条件:含水量w0即cH2O/cCTAB为13,酶液pH值为7.8,CATB浓度为0.35 mol/L,己醇浓度为13%.
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| 关 键 词： | 反胶束 固定化 醇脱氢酶 |
Preparation of Immobilized Alcohol Dehydrogenase in Reversed Micelles |
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| He Jinxing,Liu Changxian.Preparation of Immobilized Alcohol Dehydrogenase in Reversed Micelles[J].Journal of South-Central Univ for,2008,27(4). |
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| Authors: | He Jinxing Liu Changxian |
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| Abstract: | The immobilization for alcohol dehydrogenase(ADH) using reversed micelles system was studied.In the system,cetyl trimethyl ammonium bromide(CTAB) was used as surfactant,octane as solvent and hexanol as co-solvent.The effect of several factors for immobilization with ADH was investigated.The optimum conditions of immobilization with ADH were established: The w0(cH2O/cCTAB) was 13,the pH value in enzyme solution was 7.8,the concentration of CTAB and hexanol were 0.35 mol/L and 13 % respectively. |
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| Keywords: | reversed micelles immobilization alcohol dehydrogenase |
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