| Isolation and Characterization of Phytoene Desaturase cDNA from Stigma of Crocus sativus |
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| 作者姓名： | 白洁 Xu Ying Tang Lin Zeng Yu Feng Yun Wang Shenghua Chen Fang |
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| 作者单位： | [1]CollegeofLifeSciences,SichuanUniversity,Chengdu610064,P.R.China [2]CollegeofBasicScienceandForensicScience,SichuanUniversity,Chengdu610064,P.R.China |
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| 基金项目： | SupportedbytheYoungScienceFoundationofSichuanUniversity，theDoctoralFoundationoftheMinistryofEducationofChina，theSupportingProjectfortheKeyResearchProjectofSichuanProvince . |
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| 摘 要： | Phytoene desatumse (PDS) has recently been identified as an important enzyme in carotenoid biosynthesis pathway. A cDNA clone encoding phytoene desaturase gene is isolated from stigma of saffron (Crocus sativus L. ) using RT-PCR technique. Sequence analysis shows 85% similarity to Narcissus pseudonarcissus, 79% to Zea mays, 78% to Arabidopsis thaliana, 77% to Lycopersicon esculentum. A new full-length cDNA is obtained by 5 ‘-RACE and 3‘ -RACE techniques. The cDNA is 2149bp long with an open reading frame of 1697bp, which encodes a polypeptide of 565 amino acids. Southern analy-sis shows that the PDS gene is a single copy in saffron. Northern blot analysis shows higher expression level of PDS gene in stigma and anther than in leaves and stem.
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| 关 键 词： | 八氢番茄红素去氢酶基因 藏红花 分子克隆 气孔 基因表达 cDNA 类胡萝卜素 |
Isolation and Characterization of Phytoene Desaturase cDNA from Stigma of Crocus sativus |
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| Bai Jie ,Xu Ying ,Tang Lin ,Zeng Yu ,Feng Yun ,Wang Shenghua ,Chen Fang.Isolation and Characterization of Phytoene Desaturase cDNA from Stigma of Crocus sativus[J].High Technology Letters,2004,10(2):21-24. |
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| Authors: | Bai Jie Xu Ying Tang Lin Zeng Yu Feng Yun Wang Shenghua Chen Fang |
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| Institution: | 1. College of Life Sciences, Sichuan University, Chengdu 610064, P.R.China
2. College of Basic Science and Forensic Science, Sichuan University, Chengdu 610064, P.R.China |
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| Abstract: | Phytoene desaturase (PDS) has recently been identified as an important enzyme in carotenoid biosynthesis pathway. A cDNA clone encoding phytoene desaturase gene is isolated from stigma of saffron ( Crocus sativus L. ) using RT PCR technique. Sequence analysis shows 83% similarity to Narcissus pseudonarcissus, 79% to Zea mays, 78% to Arabidopsis thaliana, 77% to Lycopersicon esculentum. A new full length cDNA is obtained by 5' RACE and 3' RACE techniques. The cDNA is 2149bp long with an open reading frame of 1697bp, which encodes a polypeptide of 565 amino acids. Southern analysis shows that the PDS gene is a single copy in saffron. Northern blot analysis shows higher expression level of PDS gene in stigma and anther than in leaves and stem. |
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| Keywords: | saffron phytoene desaturase gene molecular cloning stigma expression |
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