| 荧光法研究苯甲酸氮芥铕配合物与DNA的相互作用 |
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| 引用本文： | 李菲,李怡,曹秋娥. 荧光法研究苯甲酸氮芥铕配合物与DNA的相互作用[J]. 云南大学学报(自然科学版), 2004, 26(5): 438-441 |
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| 作者姓名： | 李菲 李怡 曹秋娥 |
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| 作者单位： | 云南大学,化学系,云南,昆明,650091 |
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| 基金项目： | 云南大学校级基金资助项目 ( 2 0 0 2Q0 0 3GC) . |
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| 摘 要： | 在Tris-HCl(pH7.2)介质中,研究了1个新的苯甲酸氮芥铕(Ⅲ)配合物(Eu(BANM)3·H2O)与DNA相互作用的荧光性能,表明此配合物可作为DNA定量测定的荧光试剂.此外以溴化乙锭(EB)为荧光探针,对配合物与DNA的结合机理进行了探讨.结果证明DNA与配合物之间存在插入和沟结合2种非共价作用方式,配合物与DNA的平均结合常数为7.19×105.
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| 关 键 词： | 苯甲酸氮芥铕配合物 DNA 荧光法 |
| 文章编号： | 0258-7971(2004)05-0438-04 |
| 修稿时间： | 2003-12-03 |
Study on the interaction between DNA and the complex of benzoic acid nitrogen mustard with Eu( Ⅲ ) by the fluorescence method |
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| LI Fei,LI Yi,CAO Qiu-e. Study on the interaction between DNA and the complex of benzoic acid nitrogen mustard with Eu( Ⅲ ) by the fluorescence method[J]. Journal of Yunnan University(Natural Sciences), 2004, 26(5): 438-441 |
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| Authors: | LI Fei LI Yi CAO Qiu-e |
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| Affiliation: | Department of Chemistry, Yunnan University, Kunming 650091, China |
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| Abstract: | The studies on the fluorescence characteristic of the interaction between DNA and the complex of benzoic acid nitrogen mustard with Eu(Ⅲ)(Eu(BANM)3·H2O)in Tris-HCl medium (pH7.2) suggested that the complex could be used as a fluorescence reagent for the determination of DNA.Moreover the mechanism of the interaction between the complex and DNA was also investigated using EB as a fluorescence probe.The results pointed out that both the intercalation and groove binding existed in this system,and the average binding constant for the interaction of the complex and DNA was 7.19×105. |
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| Keywords: | complex of benzoic acid nitrogen mustard with Eu(Ⅲ) DNA fluorescence method |
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