纤维素酶提取漳州血柚皮总黄酮的工艺研究

以漳州血柚皮为原料,利用纤维素酶对血柚皮总黄酮进行提取,并以硝酸铝显色法测定总黄酮提取率.分别对酶用量、pH值、酶解温度、酶解时间进行单因素实验和正交试验,并通过极差、方差分析对提取过程显著影响提取率的因素进行统计分析.结果表明,纤维素酶提取血柚皮中的总黄酮的优选工艺条件为:酶用量5.5%,pH 5.2,酶解温度52℃,酶解时间为50 min,该工艺条件下血柚皮总黄酮的提取率可达1.52%.

Extraction of Total Flavonoids from Blood Pummelo Peel by Cellulase Hydrolysis

The total flavonoids from blood pummelo peel was prepared by cellulose enzyme hydrolysis,and the extraction yield was measured by coloration method with aluminum nitrate.The effects of parameters including enzyme concentration,pH,hydrolysis temperature and hydrolysis time on extraction yield were investigated by single factor test and orthogonal array design methods.And the notability difference was analyzed with the statistic method of range and variance.The results showed that the optimal conditions were as follows: the enzyme concentration was 5.5%,pH was 5.2,hydrolysis temperature was 52 ℃,and the hydrolysis time was 50 min.The extraction yield of the total flavonoids from blood pummelo peel reached up to 1.52%.