| 戊二醛改性胶原蛋白载体制备及在CHO细胞培养中的应用 |
| |
| 引用本文： | 刘颖利,王利莹,仓基勇,刘国清.戊二醛改性胶原蛋白载体制备及在CHO细胞培养中的应用[J].江西科学,2006,24(4):197-200. |
| |
| 作者姓名： | 刘颖利 王利莹 仓基勇 刘国清 |
| |
| 作者单位： | 1. 华北制药集团新药研究开发有限责任公司,河北,石家庄,050015
2. 华北制药股份有限公司,河北,石家庄,050015 |
| |
| 摘 要： | 天然胶原蛋白通过戊二醛改性试剂对其进行化学改性处理,继之以湿热灭菌处理以改善胶原蛋白理化性质,特别是胶原蛋白的收缩温度和膜通透性来制备动物细胞培养载体,综合改性胶原蛋白膜载体在CHO细胞生长及目的产物EPO表达方面的优势,确定新型胶原蛋白膜载体性价比优于C-DISK及进口A-DISK载体。
|
| 关 键 词： | 动物细胞培养 胶原蛋白 膜 改性 载体 |
| 文章编号： | 1001-3679(2006)04-0197-04 |
| 收稿时间： | 2006-05-30 |
| 修稿时间： | 2006-07-02 |
Preparation of Collagen Membrane Carrier Denatured by Glutaraldehyde and its Application in CHO Cell Culture |
| |
| LIU Ying-li,WANG Li-ying,CANG Ji-yong,LIU Guo-qing.Preparation of Collagen Membrane Carrier Denatured by Glutaraldehyde and its Application in CHO Cell Culture[J].Jiangxi Science,2006,24(4):197-200. |
| |
| Authors: | LIU Ying-li WANG Li-ying CANG Ji-yong LIU Guo-qing |
| |
| Abstract: | A natural collagen membrane denatured by glutaraldehyde and treated by heating keeps its chemical stability,and has better surface properties.Moreover,it is resistant against stream sterilization,so that makes it to be available for carriers in cell culture.Although the denatured collagen carrier,C-DISK,and A-DISK all are available for carrier of cell culture,both the denatured collagen carrier and C-DISK carrier are much cheaper than imported ADISK carriers,the denatured collagen carrier can still be regarded as the best carrier for cell growth and protein expression. |
| |
| Keywords: | Animal cell culture Collagen Membrane Denature Carrier |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
