PEG/磷酸盐双水相系统萃取BSA的研究

以PEG／磷酸盐双水相系统萃取BSA为对象，研究了双水相系统成相浓度、外加盐NaCl,BSA起始浓度等对BSA在两相间的分配系数和BSA下相萃取率的影响，结果发现，BSA在部分被萃取入双水相体系的下相，成相组成的PEG6000 10％（质量分数）／PO4^3- 6％（质量分数）和PEG10000 6％（质量分数／PO4^3- 6％（质量分数）的双水相系统有利于BSA的萃取分配；外加NaCl对PEG6000 10％（质量分数）／PO4^3- 6％（质量分数）系统萃取BSA的影响不大；得出了上、下相BSA浓度和BSA在两相间分配系数K与系统BSA起始浓度之间的表达式，与实验结果有较好的拟合。

Partitioning of Bovine Serum Albumin in Polyethylene Glycol(PEG)/Phosphate Aqueous Two-Phase System

BSA partitioning was performed in the PEG/phosphate aqueous two-phase system. The effects of the phase compositions concentrations, addition of NaCl and the initial concentration of BSA on the partition coefficient K and the fraction of BSA in the bottom phase Y were investigated. The results showed that BSA was enriched to the bottom phase. The phase compositions of PEG 6000 mass fraction is 10%/PO 3- 4 mass fraction is 6% and PEG 10000 mass fraction is 6%/PO 3- 4 mass fraction is 6% respectively were desirable for partitioning. The addition of NaCl showed no influence on BSA partitioning in PEG 6000 mass fraction is 10% /PO 3- 4 mass fraction is 6% system. The mathematical model for K was derived from the initial mass concentration of BSA and well fitting with the experimental data.