| 枯萎病尖孢镰刀菌的RAPD-PCR多态性分析 |
| |
| 引用本文： | 曹宜,刘波,林营志,朱育菁,周涵韬.枯萎病尖孢镰刀菌的RAPD-PCR多态性分析[J].厦门大学学报(自然科学版),2004,43(Z1):74-79. |
| |
| 作者姓名： | 曹宜 刘波 林营志 朱育菁 周涵韬 |
| |
| 作者单位： | 1. 福建省农业科学院生物技术中心,福建,福州,350003
2. 福建省农业科学院生物技术中心,福建,福州,350003;厦门大学生命科学学院,福建,厦门,361005 |
| |
| 基金项目： | 国家863计划项目(2002AA244031 2)，福建省科技厅重大项目(2000Z031)，福建省计委项目(闽计农经[2002]48号) |
| |
| 摘 要： | 利用随机扩增多态性DNA(RAPD PCR),对采自我省不同地区、不同寄主和同一寄主不同植株的瓜类枯萎病原菌尖孢镰刀菌进行遗传多样性分析,筛选到6个多态性随机引物,共产生了72条RAPD带,其中86.11%具有多态性;通过聚类分析,将供试菌株分为4个RAPD组,确定了菌株间的亲缘关系,为瓜类枯萎病原菌尖孢镰刀菌的分类提供有利的分子证据.
|
| 关 键 词： | 尖孢镰刀菌 枯萎病原菌 RAPD分析 |
| 文章编号： | 0438-0479(2004)S-074-06 |
| 修稿时间： | 2004年5月10日 |
RAPD-PCR Analysis of the Strains of Fusarium oxyporum Isolated from Wilt Diseases in the Different Crops |
| |
| CAO Yi,LIU Bo.RAPD-PCR Analysis of the Strains of Fusarium oxyporum Isolated from Wilt Diseases in the Different Crops[J].Journal of Xiamen University(Natural Science),2004,43(Z1):74-79. |
| |
| Authors: | CAO Yi LIU Bo |
| |
| Institution: | CAO Yi~1,LIU Bo~ |
| |
| Abstract: | Random amplified polymorphic DNA(RAPD)markers were used to assess genetic diversity among the strains of Fusarium oxyporum isolated from different crops. A total of 22 strains isolated from Fujian province were amplified with 6 decamer primers. As the result, 72 RAPD markers were obtained and 86.11% were polymorphisms found in the tested strains. Genetic relationships among the strains were determined by hierarchical cluster method to obtain 4 groups of RAPD, in which the relations among the strains demonstrated and discussed. RAPD analysis could provide reliable molecular evidences of classification position of F.oxyporum. |
| |
| Keywords: | Fusarium oxyporum wilt diseases RAPD analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
