| 几株花生根瘤菌的内源质粒及其与吸氢酶关联 |
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| 引用本文： | 刘红,许良树.几株花生根瘤菌的内源质粒及其与吸氢酶关联[J].厦门大学学报(自然科学版),1991,30(2):229-230. |
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| 作者姓名： | 刘红 许良树 |
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| 作者单位： | 厦门大学生物学系
(刘红，许良树，曾定)，厦门大学生物学系(张凤章) |
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| 摘 要： | 近年来,根瘤菌吸氢酶基因的研究取得可喜进展,豌豆根瘤菌(Rhizobium lequminosarum)的吸氢基因已证实与结瘤基因连锁共同编码于大质粒上。慢生型大豆根瘤菌(R.japonicum)的吸氢基因则由染色体编码,并进行基因结构和核苷酸序列的分析。花生根瘤菌(R.Trachis)内源质粒的存在情况未见报道。本工作以某些具有吸氢能力(Hup~+)和不具吸氢能力(Hup~-)的菌株为研究对象,采用Hirsch和倒转停顿电场凝胶电泳(FIGE)等方法,检测花生根瘤菌内源
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| 关 键 词： | 根瘤菌 花生 质粒 吸氢酶 基因 |
Preliminary Studies on the Relationship between H_2- uptaking Gene and Plasmid DNA from some Strains of Rhizobinm arachis |
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| Liu Hong Xu Liangshu Zeng Ding Zhang Fengzhang.Preliminary Studies on the Relationship between H_2- uptaking Gene and Plasmid DNA from some Strains of Rhizobinm arachis[J].Journal of Xiamen University(Natural Science),1991,30(2):229-230. |
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| Authors: | Liu Hong Xu Liangshu Zeng Ding Zhang Fengzhang |
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| Institution: | Department of Biology |
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| Abstract: | The plasmids of R. arachis strains have been detected with Hirsch' s and modified Ekhardt-Field Inversion Gel Electrophoresis (FIGE) methods. All of strains detected contained plasmids. Both L8-3 and its mutants showed the same plasmid profiles. Hybridizing with hup probes from pHVT 116, the 50 MD and 180 MD plasmids of strain F14-2 showed negative reaction. Thus we postulated that H2-uptaking gene was probably encoded in the chromosome or in the large plasmid of 600 MD. |
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| Keywords: | Rhizobium arachis plasmid H2-uptaking mutant molecular hybridization |
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