稻瘟病菌粗毒素对水稻成熟胚愈伤组织诱导的影响及抗性筛选

利用稻瘟病不同生理小种菌株的液体培养提取的粗毒素对水稻成熟胚进行处理,粗毒素液对水稻胚芽的生长和胚的诱导有严重的抑制作用,与对照相比差异性极显著;不同浓度的粗毒素提取液其作用效果不同,随着毒素浓度的增大或是作用时间性的延长,水稻诱导受到的抑制作用越强.水稻成熟胚组织培养中进行抗稻瘟病菌突变体筛选的毒素浓度在30%较好;浸泡水稻成熟胚以处理72h愈伤诱导和分化效果较好;在进行抗稻瘟病突变体筛选中,以用含毒素的培养基进行诱导愈伤的处理的筛选取效果较好.

The Infention of Mature Embryo Callus'S Induction by the Crude Toxin of Phyricularis Oryzae. Cav and Blast-Resistant Mutant-Screening

Mature embryos were treated by crude toxin which was extracted from liquid of different physiological races of rice blast fungus, the inhibition effect of liquid of crude toxin on the growth of rice germ and the induction of embryos was significant. Contrasted with CK, the results were significant different, and the result was different with toxin concentration changing.With the increasing of the toxin concentration and the prolonging of impact time, the inhibiting was stronger.30% was the better toxin concentration when screening of rice blast - resistant mutant from the mature embryo culture;72h was the better time to mutant induction land differentiation when the mature embryo was immersed; Contrasted the methods of screening of rice blast- resistant mutant, using the media including toxin to induct mutant was a better method.