| 一株拮抗稻瘟病内生霉菌OsiSh-10的筛选与鉴定 |
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| 引用本文： | 廖红东,袁珊珊,杨远柱,刘选明,徐 婷,胡小纯,曾夏冬,张 鑫,刘雨晴,朱咏华. 一株拮抗稻瘟病内生霉菌OsiSh-10的筛选与鉴定[J]. 湖南大学学报(自然科学版), 2015, 42(12): 80-87 |
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| 作者姓名： | 廖红东 袁珊珊 杨远柱 刘选明 徐 婷 胡小纯 曾夏冬 张 鑫 刘雨晴 朱咏华 |
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| 作者单位： | （1.湖南大学 生物学院, 湖南 长沙 410082; 2.湖南省亚华种业研究院, 湖南 长沙 410116) |
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| 摘 要： | 从湖南浏阳大围山种植的湘矮早7号水稻中，筛选到一株对水稻稻瘟病菌有显著抑制效果的水稻内生放线菌OsiSh-10菌株.由拮抗菌包覆种子和喷晒水稻叶面的实验表明将该菌喷晒于水稻叶面可以对叶瘟起到明显的控制效果，且喷一次的效果比喷两次的效果好，包覆种子反而使病情指数升高.根据形态特征、培养特征、生理生化、16s rRNA序列比对及进化树构建鉴定OsiSh-10菌株属于白色链霉菌(Streptomyces albus).OsiSh-10是一株具有稻瘟病生防潜力的菌株.
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| 关 键 词： | 鉴定；内生放线菌；稻瘟病；筛选；白色链霉菌 |
Screening and Indentification of an Endophytic Streptomyces to Antagonize Rice Blast |
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| LIAO Hong-dong,YUAN Shan-shan,YAN Yuan-zhu,LIU Xuan-ming,XU Ting,HU Xiao-chun,ZENG Xia-dong,ZHANG Xin,LIU Yu-qing,ZHU Yong-hua. Screening and Indentification of an Endophytic Streptomyces to Antagonize Rice Blast[J]. Journal of Hunan University(Naturnal Science), 2015, 42(12): 80-87 |
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| Authors: | LIAO Hong-dong YUAN Shan-shan YAN Yuan-zhu LIU Xuan-ming XU Ting HU Xiao-chun ZENG Xia-dong ZHANG Xin LIU Yu-qing ZHU Yong-hua |
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| Affiliation: | (1.College of Biology, Hunan Univ, Changsha,Hunan 410082, China;2.Hunan Yahua Seed Scientific Research Institute, Changsha,Hunan 410116, China) |
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| Abstract: | An endophytic actinomycete strain OsiSh-10 significantly against rice blast pathogens was successfully isolated from Xiangaizao 7 rice (Oryza indica) of Liuyang Dawei Mountain in Hunan Province. The control effects of OsiSh-10 on leaf blast in field were investigated by coating seeds and spraying leaf of rice with it, which were as follows: leaf blast was controlled by spraying OsiSh-10 on leaf of rice , but coated seeds with OsiSh-10 unworked for leaf blast. According to its morphological features, culture characteristics, physiological and biochemical characteristics, 16s rRNA gene sequence comparison, and phylogenetic tree construction analysis, OsiSh-10 strain was classified as Streptomyces albus. OsiSh-10 strain is a promising biocontrol actinomycete for rice blast. |
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| Keywords: | identification endophytic actinomycetes rice blast screening streptomyces albus |
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