制药废水厂微生物群落和多种抗性基因相关性分析

为了研究多种生物处理工艺制药废水中微生物群落结构和抗性基因的分布特征、扩增情况及其相关性,采用Miseq高通量测序分析技术和荧光定量PCR技术对制药废水厂中活性污泥进行检测。荧光定量结果表明:sul1,sul2,tetO,tetQ,tetW,OXA-1和可移动遗传元件int1在制药废水厂中各个阶段均能被广泛地检测到,总抗性基因浓度范围为3.09×10~8～2.26×10~9 copies/g(干重),基因总浓度上升了7.3倍。Miseq测序结果表明:制药废水中主要优势菌门为Proteobacteria,Bacteroidetes,Firmicutes,Thermus和Gemmatimonadetes等门,其平均总相对丰度比例占到81.05%;冗余分析显示,Aeromicrobium与sul2呈较高程度的正相关性,可能是sul2在微生物群落中存在的可能的主要菌群;Rhodovulum和Rhodospirillaceae菌属与OXA-1和tetQ呈较高程度的正相关性,这些菌属是这些ARGs分布的主要菌属;Acinetobacter与tetO和tetW呈现较高程度的正相关性,可能是tetO和tetW在微生物群落中存在的可能的主要菌属。因此,相关抗性基因的增值和分布与相关特定菌属有关,可以通过控制相关菌属的丰度来消减工业废水厂中的抗性基因。

Correlation between antibiotic resistance genes and microbial communities in pharmaceutical wastewater

In order to study the distribution, amplification and correlation of microbial community structure and resistance genes in pharmaceutical wastewater, a variety of biological treatment processes are used to treat pharmaceutical wastewater. Combining Miseq high throughput sequencing analysis technology and fluorescence quantitative PCR technology, activated sludge in pharmaceutical wastewater plants is detected. The results show that sul1, sul2, tetO, tetQ, tetW, OXA-1 and INT1 are widely detected at all stages of pharmaceutical wastewater treatment plant. The total resistance gene concentration ranges from 3.09×10⁸ to 2.26×10⁹ copies/g (dry weight), and the total gene concentration increases by 7.3 times. Miseq sequencing results show that the dominant bacteria in pharmaceutical wastewater are Proteobacteria, Bacteroidetes, Firmicutes, Thermus and Gemmatimonadetes, with an average relative abundance ratio of 81.05%. Redundancy analysis shows that Aeromicrobium is positively correlated with sul2 to a high degree, which might be sul2 existing in microbial communities. Among the possible major microflora, Rhodovulum and Rhodospirillaceae are highly positively correlated with OXA-1 and tetQ, which are the main microflora of ARGs distribution. Acinetobacter has a high degree of positive correlation with tetO and tetW, which may be the main possible microflora of tetO and tetW in microbial community. The results show that appreciation and distribution of antibiotic resistance gens are connected with concerned specific bacterial genus. The controlled abundance of concerned bacterial genus could subduce resistance gene in industrial wastewater factory.