| Microarray-Based Differential Expression Monitoring of 79 Novel Genes in Human Fetal Tissues |
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| 作者姓名： | Ma Shu\|hua Wang Dun\|cheng Shen Bei\|fen Wang Sheng\|qi . Institute of Radiation Medicine Academy of Military Medical Sciences B eijing . Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing |
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| 作者单位： | Ma Shu\|hua 1，Wang Dun\|cheng 2，Shen Bei\|fen 2，Wang Sheng\|qi 1 1. Institute of Radiation Medicine，Academy of Military Medical Sciences，B eijing，100850 2. Institute of Basic Medical Sciences，Academy of Military Medical Sciences，Beijing，100850 |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina(398890 0 1 ) |
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| 摘 要： | 0 ＩｎｔｒｏｄｕｃｔｉｏｎＬａｒｇｅ ｓｃａｌｅｓｅｑｕｅｎｃｉｎｇａｎｄｔｈｅａｐｐｌｉｃａｔｉｏｎｏｆｅｘｐｒｅｓｓｅｄｓｅｑｕｅｎｃｅｔａｇ(ＥＳＴ)ｔｅｃｈｎｏｌｏｇｙｈａｓｌｅｄｔｏｔｈｅｉｄｅｎｔｉｆｉｃａｔｉｏｎｏｆｈｕｎｄｒｅｄｓａｎｄｔｈｏｕｓａｎｄｓｏｆｇｅｎｏｍｉｃｓｅｑｕｅｎｃｅｓｆｒｏｍｖａｒｉｏｕｓｍｉｃｒｏｂｅｓｔｏｍｕｌｔｉｃｅｌｌｕｌａｒｅｕｋａｒｙｏｔｅｓａｎｄｐｌａｎｔｓ1,2 ] .ＥｘｐｒｅｓｓｉｏｎａｎａｌｙｓｉｓｏｆｅａｃｈｋｎｏｗｎｇｅｎｅｏｒＥＳＴｕｓｉ…
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Microarray-based differential expression monitoring of 79 novel genes in human fetal tissues |
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| Ma?Shu-hua,Wang?Dun-cheng,Shen?Bei-fen,Wang?Sheng-qi.Microarray-Based Differential Expression Monitoring of 79 Novel Genes in Human Fetal Tissues[J].Wuhan University Journal of Natural Sciences,2003,8(1):125-129. |
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| Authors: | Ma Shu-hua Wang Dun-cheng Shen Bei-fen Wang Sheng-qi |
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| Institution: | (1) Institute of Radiation Medicine, Academy of Military Medical Sciences, 100850 Beijing;(2) Institute of Basic Medical Sciences, Academy of Military Medical Sciences, 100850 Beijing |
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| Abstract: | ESTs fragments which represents corresponding novel genes were obtained by sequencing and bioinformatics analysis of human fet al kidney cDNA library. Microarray was prepared by using these novel EST fragmen ts by automatic spotting. Expression patters of 79 ESTs of novel genes from huma n fetal kidney were analyzed in fetal brain and fetal heart tissues of 20\|week\ | and 26\|week\|age fetus by performing of cDNA chip hybridization. This provide s clues for studying exact functions of the novel genes. 8 genes were obtained w hich were expressed differentially in the fetal brain and heart of 20\|week\| an d 26\|week\|age respectively. Then differentially expressed genes were identifie d by Northern analysis. The more exact function of the novel genes is under stud y. |
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| Keywords: | cDNA chip novel genes expressed sequence t ag (EST) differential expression Northern analysis |
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