| 复合酶法提取红托竹荪多糖的工艺研究 |
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| 引用本文： | 叶敏.复合酶法提取红托竹荪多糖的工艺研究[J].吉林师范大学学报（自然科学版）,2012(2):145-147. |
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| 作者姓名： | 叶敏 |
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| 作者单位： | 毕节学院化学与化工学院 |
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| 基金项目： | 贵州省毕节学院科学研究基金项目 |
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| 摘 要： | 采用复合酶法提取红托竹荪中的水溶性多糖,通过单因素试验和正交试验研究酶浓度、酶解时间、酶解温度以及酶解pH值对多糖得率的影响.确定复合酶法提取红托竹荪水溶性多糖的最佳条件为:酶浓度为1.5%,酶解时间120 min,酶解温度50℃,酶解pH值为4.5,此条件下的红托竹荪多糖得率为7.98%.
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| 关 键 词： | 红托竹荪 多糖 复合酶 |
Study on Composite Enzymatic Extraction of D.rubrovalvata Polysaccharides |
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| YE Min.Study on Composite Enzymatic Extraction of D.rubrovalvata Polysaccharides[J].Jilin Normal University Journal(Natural Science Edition),2012(2):145-147. |
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| Authors: | YE Min |
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| Institution: | YE Min (School of Chemistry and Chemical Engineering,Bijie University,Bijie 551700,China) |
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| Abstract: | Composite enzymatic(cellulase,protease and pectase) extraction of D.rubrovalvata polysaccharides was studied in this paper.The main factors that affect extraction yield in the final product were analyzed,such as enzyma reaction temperature,pH,time and enzyma concentration.By means of single factor experiment and orthogonal design,optimum parameters were confirmed as enzyma reaction temperature 50℃,enzyma reaction pH4.5,enzyma reaction time 120 min and enzyma concentration 1.5%.The extraction yield of D.rubrovalvata polysaccharide is 7.98% under the above conditions. |
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| Keywords: | D rubrovalvata polysaccharides composite enzyme |
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